ArticleOptics express2019
Adaptive optics allows STED-FCS measurements in the cytoplasm of living cells.
Article in Optics express, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Minimal differences observed when comparing the morphological profiling of microglia obtained by confocal laser scanning and optical sectioning microscopy.Frontiers in neuroanatomy · 2024Article
- Precision isolation and cultivation of single cells by vortex and flat-top laser ejection.Frontiers in microbiology · 2024Article
- Current capabilities and future perspectives of FCS: super-resolution microscopy, machine learning, and in vivo applications.Communications biology · 2023Review
- Diffusion and interaction dynamics of the cytosolic peroxisomal import receptor PEX5.Biophysical reports · 2022Article
- Laser induced forward transfer isolating complex-shaped cell by beam shaping.Biomedical optics express · 2021Article
- Aberration correction in stimulated emission depletion microscopy to increase imaging depth in living brain tissue.Neurophotonics · 2021Article
- Chemically-powered swimming and diffusion in the microscopic world.Nature reviews. Chemistry · 2021Review
- Article
- Advances in Confocal Microscopy and Selected Applications.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Practical sensorless aberration estimation for 3D microscopy with deep learning.Optics express · 2020Article
- Background Reduction in STED-FCS Using a Bivortex Phase Mask.ACS photonics · 2020Article
- Article
- z-STED Imaging and Spectroscopy to Investigate Nanoscale Membrane Structure and Dynamics.Biophysical journal · 2020Article
- High photon count rates improve the quality of super-resolution fluorescence fluctuation spectroscopy.Journal of physics D: Applied physics · 2020Article
- Fluorescence Microscopy of the HIV-1 Envelope.Viruses · 2020Review
- Cellular Membranes, a Versatile Adaptive Composite Material.Frontiers in cell and developmental biology · 2020Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Fluorescence correlation spectroscopy in combination with super-resolution stimulated emission depletion microscopy (STED-FCS) is a powerful tool to investigate molecular diffusion with sub-diffraction resolution. It has been of particular use for investigations of two dimensional systems like cell membranes, but has so far seen very limited applications to studies of three-dimensional diffusion. One reason for this is the extreme sensitivity of the axial (z) STED depletion pattern to optical aberrations. We present here an adaptive optics-based correction method that compensates for these aberrations and allows STED-FCS measurements in the cytoplasm of living cells.
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Registered trials
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