Evidence map›Paper›PMID 31508984›Full record

ArticleAnnals of the Royal College of Surgeons of England2019

A simple ex vivo model of human renal allograft preservation using the gonadal vein.

W P Ries, Y Marie, K Patel, C Turnbull, T B Smith, Nsm Jamil, H Caldwell, R Telfer, Dah Neil, J Nath and 1 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Annals of the Royal College of Surgeons of England, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 92% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 1 country.

W P RiesMedical School, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.
Y MarieDepartment of Renal Surgery, Queen Elizabeth Hospital, University Hospitals Birmingham NHS Foundation Trust, Birmingham, UK.
K PatelDepartment of Renal Surgery, Queen Elizabeth Hospital, University Hospitals Birmingham NHS Foundation Trust, Birmingham, UK.
C TurnbullMedical School, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.
T B SmithInstitute of Metabolism and Systems Research, University of Birmingham, Birmingham, UK.
Nsm JamilDepartment of Cellular Pathology, Royal Victoria Infirmary, Durham and Darlington NHS Foundation Trust, Newcastle Upon Tyne, UK.
H CaldwellDivision of Pathology Laboratories, University of Edinburgh, Western General Hospital, NHS Lothian, Edinburgh, UK.
R TelferMedical School, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.
Dah NeilDepartment of Histopathology, Queen Elizabeth Hospital, University Hospitals Birmingham NHS Foundation Trust, Birmingham, UK.
J NathDepartment of Renal Surgery, Queen Elizabeth Hospital, University Hospitals Birmingham NHS Foundation Trust, Birmingham, UK.
N G InstonDepartment of Renal Surgery, Queen Elizabeth Hospital, University Hospitals Birmingham NHS Foundation Trust, Birmingham, UK.
University of Birmingham · GBQueen Elizabeth Hospital · GBUniversity Hospitals Birmingham NHS Foundation Trust · GBRoyal Victoria Infirmary · GBUniversity of Edinburgh · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionHypothermic machine perfusion, an organ preservation modality, involves flow of chilled preservation fluid through an allograft's vasculature. This study describes a simple, reproducible, human model that allows for interrogation of flow effects during ex vivo organ perfusion. MATERIALS AND

methodsGonadal veins from deceased human renal allografts were subjected to either static cold storage or hypothermic machine perfusion for up to 24 hours. Caspase-3, Krüppel-like factor 2 expression and electron microscopic analysis were compared between 'flow' and 'no-flow' conditions, with living donor gonadal vein sections serving as negative controls.

resultsThe increase in caspase-3 expression was less pronounced for hypothermic machine-perfused veins compared with static cold storage (median-fold increase 1.2 vs 2.3;

conclusionsOur methodology is a simple, reproducible and successful model of ex vivo perfusion in the context of human organ preservation. To demonstrate the model's utility, we establish that two widely used markers of endothelial health (caspase-3 and Krüppel-like factor 2) differ between the flow and no-flow conditions of the two predominant kidney preservation modalities. These findings suggest that ex vivo perfusion may mediate the induction of a biochemically favourable endothelial niche which may contribute tohypothermic machine perfusion's association with improved renal transplantation outcomes.

Indexed as

Models, BiologicalApoptosisBiomarkersCadaverCaspase 3Cold TemperatureEndothelium, VascularHumansKidneyKidney TransplantationKruppel-Like Transcription FactorsMicroscopy, ElectronOrgan PreservationOrgan Preservation SolutionsPerfusionVeinsBiomarkersCaspase 3KLF3 protein, humanKruppel-Like Transcription FactorsOrgan Preservation SolutionsKidney transplantationKrüppel-like transcription factorsOrgan preservation

Identifiers

PMID31508984
PMCPMC6818055
OpenAlexW2972982516

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.