Stiffness of Cargo-Motor Linkage Tunes Myosin VI Motility and Response to Load.

Rachit Shrivastava, Ashim Rai, Murti Salapaka, Sivaraj Sivaramakrishnan

Abstract read

In one paragraph

Article in Biochemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Rachit ShrivastavaDepartment of Electrical and Computer Engineering , University of Minnesota Twin Cities , Minneapolis , Minnesota 55455 , United States.

Ashim RaiDepartment of Genetics, Cell Biology, and Development , University of Minnesota Twin Cities , Minneapolis , Minnesota 55108 , United States.

Murti SalapakaDepartment of Electrical and Computer Engineering , University of Minnesota Twin Cities , Minneapolis , Minnesota 55455 , United States.

Sivaraj SivaramakrishnanDepartment of Genetics, Cell Biology, and Development , University of Minnesota Twin Cities , Minneapolis , Minnesota 55108 , United States.ORCID 0000-0002-9541-6994

Funding

Emergent cellular functions of GPCRs and myosinsR35GM126940 · NIGMS · UNIVERSITY OF MINNESOTA · PI Sivaraj Sivaramakrishnan · 2018 to 2026

$3.8M

NIGMS NIH HHS R35 GM126940

6 · The paper itself

Abstract

We examine the effect of cargo-motor linkage stiffness on the mechanobiological properties of the molecular motor myosin VI. We use the programmability of DNA nanostructures to modulate cargo-motor linkage stiffness and combine it with high-precision optical trapping measurements to measure the effect of linkage stiffness on the motile properties of myosin VI. Our results reveal that a stiff cargo-motor linkage leads to shorter step sizes and load-induced anchoring of myosin VI, while a flexible linkage results in longer steps with frequent detachments from the actin filament under load. Our findings suggest a novel regulatory mechanism for tuning the dual cellular roles of the anchor and transporter ascribed to myosin VI.

Indexed as

Actin CytoskeletonAnimalsBiomechanical PhenomenaDNAHumansMolecular Motor ProteinsMyosin Heavy ChainsNanostructuresOptical TweezersPliabilityDNAMolecular Motor ProteinsMyosin Heavy Chainsmyosin VI

Identifiers

PMID31508940

DOI10.1021/acs.biochem.9b00422

PMCPMC7055456

What OpenQuestion holds

Textmetadata

LicenceTDM

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.