Evidence map›Paper›PMID 31506763›Full record

ArticleUrolithiasis2020

miRNA-34a inhibits cell adhesion by targeting CD44 in human renal epithelial cells: implications for renal stone disease.

Bohan Wang, Gaofei He, Gang Xu, Jiaming Wen, Xiao Yu

Abstract read
PubMed Publisher
In one paragraph

Article in Urolithiasis, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed, 1 pooled it
0.8field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 1 synthesis or guideline pooled it, 27 citations in OpenAlex.

  1. Pooled it
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  4. Review
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  12. Review
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  15. Noncoding RNA, friend or foe for nephrolithiasis?Frontiers in cell and developmental biology · 2024
    Review
  16. Article
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  20. Protective Effect of DegradedOxidative medicine and cellular longevity · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Bohan WangDepartment of Urology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Zhejiang University, Hangzhou, China.
Gaofei HeDepartment of Urology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Zhejiang University, Hangzhou, China.
Gang XuDepartment of Urology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Zhejiang University, Hangzhou, China.
Jiaming WenDepartment of Urology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Zhejiang University, Hangzhou, China. wenjiaming@zju.edu.cn.
Xiao YuDepartment of Urology, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, China. yujiuhu8366@126.com.
Second Affiliated Hospital of Zhejiang University · CNTongji Hospital · CN

Funding

National Natural Science Foundation of China 81500532National Natural Science Foundation of China 81871153Natural Science Foundation of Zhejiang Province LY18H040007
6 · The paper itself

Abstract

Nephrolithiasis is a very common disease in which cell-crystal adhesion is an essential mechanism for kidney stone formation. This study has explored the anti-adhesion function of the microRNA, miR-34a, by targeting CD44, a cell surface receptor, in human renal epithelial (HK-2) cells. The expression of CD44 was monitored by qPCR and western blot. A luciferase assay validated the target of miR-34a in CD44 3' UTR. Immunofluorescence staining under confocal microscopy was used to detect the cell-crystal adhesion effects in vitro. Pizzolato staining was performed to examine the adhesion role of miR-34a in vivo. In HK-2 cells, miR-34a was down-regulated and CD44 was up-regulated when exposed to calcium oxalate monohydrate crystals. Moreover, miR-34a negatively regulated the expression of CD44. According to the luciferase report assay, miR-34a direct targeted a binding site in the CD44 3'UTR. In vitro experiments, miR-34a overexpression inhibited CD44 expression and cell-crystals adhesion; whereas CD44 overexpression showed reversed results. Furthermore, miR-34a suppressed cell-crystals adhesion and stone formation in vivo. These findings indicate that miR-34a targets CD44 in HK-2 cells and inhibits cell-crystal adhesion both in vitro and in vivo. Based on these results, miR-34a may be a potential therapeutic target for renal stone disease.

Indexed as

Calcium OxalateCell AdhesionCell LineDown-RegulationEpithelial CellsGene Expression ProfilingHumansHyaluronan ReceptorsKidney CalculiMicroRNAsReal-Time Polymerase Chain ReactionCalcium OxalateCD44 protein, humanHyaluronan ReceptorsMicroRNAsMIRN34 microRNA, humanCD44 proteinCell adhesionKidney stone diseaseMicroRNA

Identifiers

PMID31506763
OpenAlexW2973037640

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.