ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2020
Evaluation of a laboratory-developed test for simultaneous detection of norovirus and rotavirus by real-time RT-PCR on the Panther Fusion® system.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 18 citations in OpenAlex.
- Development of a Real-Time Polymerase Chain Reaction Assay to DetectDiagnostics (Basel, Switzerland) · 2026Article
- Evaluation of Universal Biomolecular Medium for Molecular Detection of Respiratory Viruses: A Paired, Multi-Platform Comparison.Microorganisms · 2026Article
- Adaptation and validation of a gastrointestinal panel to detect diarrheal virus pathogens on a high-throughput qPCR system.Medical microbiology and immunology · 2025Article
- Screening forBio-protocol · 2025Article
- The Abnormal Accumulation of Lipopolysaccharide Secreted by Enriched Gram-Negative Bacteria Increases the Risk of Rotavirus Colonization in Young Adults.Microorganisms · 2023Article
- Optimization and Clinical Evaluation of an Automated Commercial Analyte-Specific Reagent Assay forJournal of clinical microbiology · 2023Article
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- Crosstalk between imbalanced gut microbiota caused by antibiotic exposure and rotavirus replication in the intestine.Heliyon · 2023Article
- Current State and Future Perspectives on Personalized Metabolomics.Metabolites · 2023Review
- rprimer: an R/bioconductor package for design of degenerate oligos for sequence variable viruses.BMC bioinformatics · 2022Article
- Multiplex detection of eight different viral enteropathogens in clinical samples, combining RT-PCR technology with melting curve analysis.Virology journal · 2022Article
- Metabolomic Laboratory-Developed Tests: Current Status and Perspectives.Metabolites · 2021Review
- Holistic Metabolomic Laboratory-Developed Test (LDT): Development and Use for the Diagnosis of Early-Stage Parkinson's Disease.Metabolites · 2020Article
- Diagnosis of Parkinson's Disease by A Metabolomics-Based Laboratory-Developed Test (LDT).Diagnostics (Basel, Switzerland) · 2020Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Hologic Panther Fusion® Open Access™ functionality allows implementation of laboratory-developed tests (LDTs), with fully automated sample extraction, real-time PCR, and result interpretation. We report the development and validation of a multiplex LDT for norovirus G1, norovirus G2, and rotavirus from stool samples on this system. The LDT was optimized for primer and probe sequences, salt concentration, and PCR annealing temperature. Reproducibility of the PCR and extraction process was assessed. Performance of the multiplex LDT assay was evaluated with external quality assessment (EQA) samples and compared to a commercial multiplex assay (Allplex™ GI-Virus Assay, Seegene) in clinical samples. Salt concentrations and annealing/extension temperature were optimized to 4 mM MgCl
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