ArticleScientific reports2019
Dynamics of centriole amplification in centrosome-depleted brain multiciliated progenitors.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 42 citations in OpenAlex.
- Cerebrospinal fluid-driven ependymal motile cilia defects are implicated in multiple sclerosis.Brain : a journal of neurology · 2026Article
- Crashing by design: Utilizing DNA damage for MCC differentiation.Trends in cell biology · 2026Article
- The cell cycle variant in multiciliated cells incorporates 2 centriole biogenesis cycles.bioRxiv : the preprint server for biology · 2026Article
- DEUP1 functions as a scaffold for basal foot integrity and planar polarity in multiciliated cells.Nature communications · 2026Article
- Repurposing Cell Cycle Oscillators for Multiciliated Cell Differentiation.Advances in experimental medicine and biology · 2026Review
- Taking Down the Primary Cilium: Pathways for Disassembly in Differentiating Cells.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- The primary cilium as a multifunctional organelle: emerging roles and unanswered questions.Cell communication and signaling : CCS · 2025Review
- Formation and function of multiciliated cells.The Journal of cell biology · 2024Review
- Live-Imaging Centriole Amplification in Mouse Brain Multiciliated Cells.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Deup1 Expression Interferes with Multiciliated Differentiation.Molecules and cells · 2023Article
- Article
- Advances in Understanding the Genetic Mechanisms of Zebrafish Renal Multiciliated Cell Development.Journal of developmental biology · 2022Review
- One master to rule them all.eLife · 2022Article
- Development of a multiciliated cell.Current opinion in cell biology · 2022Review
- Article
- Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM).Nature methods · 2022Article
- Experimental and Natural Induction ofFrontiers in cell and developmental biology · 2022Review
- Plk4 triggers autonomous de novo centriole biogenesis and maturation.The Journal of cell biology · 2021Article
- A first-takes-all model of centriole copy number control based on cartwheel elongation.PLoS computational biology · 2021Article
- Biophysical and biochemical properties of Deup1 self-assemblies: a potential driver for deuterosome formation during multiciliogenesis.Biology open · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reproductive and respiratory organs, along with brain ventricles, are lined by multiciliated epithelial cells (MCC) that generate cilia-powered fluid flows. MCC hijack the centrosome duplication pathway to form hundreds of centrioles and nucleate motile cilia. In these cells, the large majority of procentrioles are formed associated with partially characterized organelles called deuterosomes. We recently challenged the paradigm that deuterosomes and procentrioles are formed de novo by providing data, in brain MCC, suggesting that they are nucleated from the pre-existing centrosomal younger centriole. However, the origin of deuterosomes and procentrioles is still under debate. Here, we further question centrosome importance for deuterosome and centriole amplification. First, we provide additional data confirming that centriole amplification occurs sequentially from the centrosomal region, and that the first procentriole-loaded deuterosomes are associated with the daughter centriole or in the centrosomal centriole vicinity. Then, to further test the requirement of the centrosome in deuterosome and centriole formation, we depleted centrosomal centrioles using a Plk4 inhibitor. We reveal unexpected limited consequences in deuterosome/centriole number in absence of centrosomal centrioles. Notably, in absence of the daughter centriole only, deuterosomes are not seen associated with the mother centriole. In absence of both centrosomal centrioles, procentrioles are still amplified sequentially and with no apparent structural defects. They seem to arise from a focal region, characterized by microtubule convergence and pericentriolar material (PCM) assembly. The relevance of deuterosome association with the daughter centriole as well as the role of the PCM in the focal and sequential genesis of centrioles in absence of centrosomal centrioles are discussed.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.