Evidence map›Paper›PMID 31506510›Full record

ArticleScientific reports2019

Species-Specific Marker Discovery in Tilapia.

Mochamad Syaifudin, Michaël Bekaert, John B Taggart, Kerry L Bartie, Stefanie Wehner, Christos Palaiokostas, M G Q Khan, Sarah-Louise C Selly, Gideon Hulata, Helena D'Cotta and 3 more

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.6field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 34 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 6 countries.

Mochamad SyaifudinInstitute of Aquaculture, University of Stirling, Stirling, Scotland, United Kingdom.ORCID http://orcid.org/0000-0002-2586-9672
Michaël BekaertInstitute of Aquaculture, University of Stirling, Stirling, Scotland, United Kingdom.ORCID http://orcid.org/0000-0002-1206-7654
John B TaggartInstitute of Aquaculture, University of Stirling, Stirling, Scotland, United Kingdom.
Kerry L BartieInstitute of Aquaculture, University of Stirling, Stirling, Scotland, United Kingdom.
Stefanie WehnerInstitute of Aquaculture, University of Stirling, Stirling, Scotland, United Kingdom.
Christos PalaiokostasInstitute of Aquaculture, University of Stirling, Stirling, Scotland, United Kingdom.
M G Q KhanInstitute of Aquaculture, University of Stirling, Stirling, Scotland, United Kingdom.
Sarah-Louise C SellyInstitute of Aquaculture, University of Stirling, Stirling, Scotland, United Kingdom.
Gideon HulataInstitute of Animal Science, Agricultural Research Organization, The Volcani Center, Rishon LeZion 7505101, Israel.
Helena D'CottaISEM, Univ. Montpellier, CNRS, IRD, EPHE, Montpellier, France.
Jean-Francois BaroillerISEM, Univ. Montpellier, CNRS, IRD, EPHE, Montpellier, France.
Brendan J McAndrewInstitute of Aquaculture, University of Stirling, Stirling, Scotland, United Kingdom.
David J PenmanInstitute of Aquaculture, University of Stirling, Stirling, Scotland, United Kingdom. d.j.penman@stir.ac.uk.
University of Stirling · GBCentre National de la Recherche Scientifique · FRAgricultural Research Organization · IL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tilapias (family Cichlidae) are of importance in aquaculture and fisheries. Hybridisation and introgression are common within tilapia genera but are difficult to analyse due to limited numbers of species-specific genetic markers. We tested the potential of double digested restriction-site associated DNA (ddRAD) sequencing for discovering single nucleotide polymorphism (SNP) markers to distinguish between 10 tilapia species. Analysis of ddRAD data revealed 1,371 shared SNPs in the de novo-based analysis and 1,204 SNPs in the reference-based analysis. Phylogenetic trees based on these two analyses were very similar. A total of 57 species-specific SNP markers were found among the samples analysed of the 10 tilapia species. Another set of 62 species-specific SNP markers was identified from a subset of four species which have often been involved in hybridisation in aquaculture: 13 for Oreochromis niloticus, 23 for O. aureus, 12 for O. mossambicus and 14 for O. u. hornorum. A panel of 24 SNPs was selected to distinguish among these four species and validated using 91 individuals. Larger numbers of SNP markers were found that could distinguish between the pairs of species within this subset. This technique offers potential for the investigation of hybridisation and introgression among tilapia species in aquaculture and in wild populations.

Indexed as

Genetic MarkersPolymorphism, Single NucleotideAnimalsHybridization, GeneticPhylogenySequence Analysis, DNASpecies SpecificityTilapiaGenetic Markers

Identifiers

PMID31506510
PMCPMC6737103
OpenAlexW2972979162

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.