Evidence map›Paper›PMID 31504847›Full record

ArticleNucleic acids research2019

mTOR-regulated U2af1 tandem exon splicing specifies transcriptome features for translational control.

Jae-Woong Chang, Hsin-Sung Yeh, Meeyeon Park, Luke Erber, Jiao Sun, Sze Cheng, Alexander M Bui, Naima Ahmed Fahmi, Ryan Nasti, Rui Kuang and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nucleic acids research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
1.0field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 19 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 1 country.

Jae-Woong ChangDepartment of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Twin Cities, Minneapolis, MN 55455, USA.
Hsin-Sung YehDepartment of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Twin Cities, Minneapolis, MN 55455, USA.
Meeyeon ParkDepartment of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Twin Cities, Minneapolis, MN 55455, USA.
Luke ErberDepartment of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Twin Cities, Minneapolis, MN 55455, USA.
Jiao SunDepartment of Computer Science, University of Central Florida, Orlando, FL 32816, USA.
Sze ChengDepartment of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Twin Cities, Minneapolis, MN 55455, USA.
Alexander M BuiDepartment of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Twin Cities, Minneapolis, MN 55455, USA.
Naima Ahmed FahmiDepartment of Computer Science, University of Central Florida, Orlando, FL 32816, USA.
Ryan NastiDepartment of Genetics, Cell and Developmental Biology, University of Minnesota Twin Cities, Minneapolis, MN 55455, USA.
Rui KuangDepartment of Computer Science and Engineering, University of Minnesota Twin Cities, Minneapolis, MN 55455, USA.
Yue ChenDepartment of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Twin Cities, Minneapolis, MN 55455, USA.
Wei ZhangDepartment of Computer Science, University of Central Florida, Orlando, FL 32816, USA.
Jeongsik YongDepartment of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Twin Cities, Minneapolis, MN 55455, USA.
University of Minnesota · USUniversity of Central Florida · US

Funding

The Role of Truncated mRNAs in CancerR01GM113952 · NIGMS · UNIVERSITY OF MINNESOTA · PI YONG, JEONGSIK · 2015 to 2023
$3.0M
NIGMS NIH HHS R01 GM113952
6 · The paper itself

Abstract

U2 auxiliary factor 1 (U2AF1) functions in 3'-splice site selection during pre-mRNA processing. Alternative usage of duplicated tandem exons in U2AF1 produces two isoforms, U2AF1a and U2AF1b, but their functional differences are unappreciated due to their homology. Through integrative approaches of genome editing, customized-transcriptome profiling and crosslinking-mediated interactome analyses, we discovered that the expression of U2AF1 isoforms is controlled by mTOR and they exhibit a distinctive molecular profile for the splice site and protein interactomes. Mechanistic dissection of mutually exclusive alternative splicing events revealed that U2AF1 isoforms' inherent differential preferences of nucleotide sequences and their stoichiometry determine the 3'-splice site. Importantly, U2AF1a-driven transcriptomes feature alternative splicing events in the 5'-untranslated region (5'-UTR) that are favorable for translation. These findings unveil distinct roles of duplicated tandem exon-derived U2AF1 isoforms in the regulation of the transcriptome and suggest U2AF1a-driven 5'-UTR alternative splicing as a molecular mechanism of mTOR-regulated translational control.

Indexed as

Protein BiosynthesisAlternative SplicingAnimalsBase SequenceExonsHeLa CellsHumansMiceRNA Splice SitesRNA SplicingSplicing Factor U2AFTOR Serine-Threonine KinasesTranscriptomeRNA Splice SitesSplicing Factor U2AFTOR Serine-Threonine KinasesZrsr1 protein, mouse

Identifiers

PMID31504847
PMCPMC6821156
OpenAlexW2971484324

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.