Evidence map›Paper›PMID 31504797›Full record

ArticleNeuro-oncology2019

CircFOXO3 promotes glioblastoma progression by acting as a competing endogenous RNA for NFAT5.

Shuai Zhang, Keman Liao, Zengli Miao, Qing Wang, Yifeng Miao, Zhongye Guo, Yun Qiu, Binghong Chen, Li Ren, Zilong Wei and 3 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuro-oncology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.

0numbers the graph read from it
0cells of the map it votes in
61citing papers in PubMed
6.1field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

61 citing papers in PubMed, 100 citations in OpenAlex.

  1. Article
  2. Article
  3. CircFoxo3 knockdown inhibit gastric cancer progression by regulating Foxo3.In vitro cellular & developmental biology. Animal · 2026
    Article
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  11. Regulation of autophagy by non-coding RNAs in human glioblastoma.Medical oncology (Northwood, London, England) · 2024
    Review
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1 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 1 country.

Shuai ZhangDepartment of Neurosurgery, the Affiliated Wuxi No. 2 People's Hospital of Nanjing Medical University, Wuxi, China.
Keman LiaoDepartment of Neurosurgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Zengli MiaoDepartment of Neurosurgery, the Affiliated Wuxi No. 2 People's Hospital of Nanjing Medical University, Wuxi, China.
Qing WangDepartment of Neurosurgery, the Affiliated Wuxi No. 2 People's Hospital of Nanjing Medical University, Wuxi, China.
Yifeng MiaoDepartment of Neurosurgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Zhongye GuoDepartment of Neurosurgery, the Affiliated Wuxi No. 2 People's Hospital of Nanjing Medical University, Wuxi, China.
Yun QiuDepartment of Neurosurgery, the Affiliated Wuxi No. 2 People's Hospital of Nanjing Medical University, Wuxi, China.
Binghong ChenDepartment of Neurosurgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Li RenDepartment of Neurosurgery, Shanghai Pudong Hospital, Fudan University, Shanghai, China.
Zilong WeiDepartment of Neurosurgery, Shanghai Pudong Hospital, Fudan University, Shanghai, China.
Yingying LinDepartment of Neurosurgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Xiaojie LuDepartment of Neurosurgery, the Affiliated Wuxi No. 2 People's Hospital of Nanjing Medical University, Wuxi, China.
Yongming QiuDepartment of Neurosurgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Shanghai Jiao Tong University · CNWuxi No.2 People's Hospital · CNFudan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCircular RNAs (circRNAs), a newly discovered type of endogenous noncoding RNA, have been proposed to mediate the progression of diverse types of tumors. Systematic studies of circRNAs have just begun, and the physiological roles of circRNAs remain largely unknown. Here, we focused on elucidating the potential role and molecular mechanism of circular forkhead box O3 (circFOXO3) in glioblastoma (GBM) progression.

methodsFirst, we analyzed circFOXO3 alterations in GBM and noncancerous tissues through real-time quantitative reverse transcription PCR (qRT-PCR). Next, we used loss- and gain-of-function approaches to evaluate the effect of circFOXO3 on GBM cell proliferation and invasion. Mechanistically, fluorescent in situ hybridization, RNA pull-down, dual luciferase reporter, and RNA immunoprecipitation assays were performed to confirm the interaction between circFOXO3 and miR-138-5p/miR-432-5p in GBM. An animal model was used to verify the in vitro experimental findings.

resultsCircFOXO3 expression was significantly higher in GBM tissues than in noncancerous tissues. GBM cell proliferation and invasion were reduced by circFOXO3 knockdown and enhanced by circFOXO3 overexpression. Further biochemical analysis showed that circFOXO3 exerted its pro-tumorigenic activity by acting as a competing endogenous RNA (ceRNA) to increase expression of nuclear factor of activated T cells 5 (NFAT5) via sponging both miR-138-5p and miR-432-5p. Notably, tumor inhibition by circFOXO3 downregulation could be reversed by miR-138-5p/miR-432-5p inhibitors in GBM cells. Moreover, GBM cells with lower circFOXO3 expression developed less aggressive tumors in vivo.

conclusionsOur data demonstrate that circFOXO3 can exert regulatory functions in GBM and that ceRNA-mediated microRNA sequestration might be a potential strategy for GBM therapy.

Indexed as

AnimalsBrain NeoplasmsDisease ProgressionDNA, CircularForkhead Box Protein O3Gene Expression Regulation, NeoplasticGlioblastomaHeterograftsHumansMiceMice, NudeMicroRNAsTranscription FactorsDNA, CircularForkhead Box Protein O3FOXO3 protein, humanMicroRNAsMIRN138 microRNA, humanMIRN432 microRNA, humanNFAT5 protein, humanTranscription FactorsceRNAcircFOXO3glioblastomamiR-138-5p/miR-432-5pNFAT5

Identifiers

PMID31504797
PMCPMC6784278
OpenAlexW2971628029

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.