ArticleJournal of experimental botany2019
The pyrenoidal linker protein EPYC1 phase separates with hybrid Arabidopsis-Chlamydomonas Rubisco through interactions with the algal Rubisco small subunit.
Article in Journal of experimental botany, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 64 citations in OpenAlex.
- EPOS1 is required for normal pyrenoid starch sheath formation and efficiency of the COThe New phytologist · 2026Article
- CIA5 and its interacting metal-binding GTPase ZNG3 are degraded by the proteasome in Zn deficiency.The Plant journal : for cell and molecular biology · 2026Article
- Phase separation of PGL-3 driven by structured domains that oligomerize and interact with RGG motifs.EMBO reports · 2026Article
- Not just Rubisco: integrated strategies to overcome the biochemical limitations of CFrontiers in plant science · 2026Review
- Reprogramming encapsulins into modular carbon-fixing nanocompartments.Nature communications · 2025Article
- Cellular view of metabolism: metabolic biomolecular condensates.The New phytologist · 2025Review
- Strategies to improve photosynthesis by modifying the RuBisCO system and its limitations.Molecular biology reports · 2025Review
- Engineering Rubisco condensation in chloroplasts to manipulate plant photosynthesis.Plant biotechnology journal · 2025Article
- Review
- Bestrophin-like protein 4 is involved in photosynthetic acclimation to light fluctuations in Chlamydomonas.Plant physiology · 2024Article
- A promiscuous mechanism to phase separate eukaryotic carbon fixation in the green lineage.Nature plants · 2024Article
- Review
- Review
- Pyrenoid proteomics reveals independent evolution of the COProceedings of the National Academy of Sciences of the United States of America · 2024Article
- SAGA1 and SAGA2 promote starch formation around proto-pyrenoids in Arabidopsis chloroplasts.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- The pyrenoid: the eukaryotic CO2-concentrating organelle.The Plant cell · 2023Article
- The small subunit of Rubisco and its potential as an engineering target.Journal of experimental botany · 2023Review
- Phase-separating pyrenoid proteins form complexes in the dilute phase.Communications biology · 2023Article
- New horizons for building pyrenoid-based CO2-concentrating mechanisms in plants to improve yields.Plant physiology · 2022Review
- The Rubisco small subunits in the green algal genus Chloromonas provide insights into evolutionary loss of the eukaryotic carbon-concentrating organelle, the pyrenoid.BMC ecology and evolution · 2021Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
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Abstract
Photosynthetic efficiencies in plants are restricted by the CO2-fixing enzyme Rubisco but could be enhanced by introducing a CO2-concentrating mechanism (CCM) from green algae, such as Chlamydomonas reinhardtii (hereafter Chlamydomonas). A key feature of the algal CCM is aggregation of Rubisco in the pyrenoid, a liquid-like organelle in the chloroplast. Here we have used a yeast two-hybrid system and higher plants to investigate the protein-protein interaction between Rubisco and essential pyrenoid component 1 (EPYC1), a linker protein required for Rubisco aggregation. We showed that EPYC1 interacts with the small subunit of Rubisco (SSU) from Chlamydomonas and that EPYC1 has at least five SSU interaction sites. Interaction is crucially dependent on the two surface-exposed α-helices of the Chlamydomonas SSU. EPYC1 could be localized to the chloroplast in higher plants and was not detrimental to growth when expressed stably in Arabidopsis with or without a Chlamydomonas SSU. Although EPYC1 interacted with Rubisco in planta, EPYC1 was a target for proteolytic degradation. Plants expressing EPYC1 did not show obvious evidence of Rubisco aggregation. Nevertheless, hybrid Arabidopsis Rubisco containing the Chlamydomonas SSU could phase separate into liquid droplets with purified EPYC1 in vitro, providing the first evidence of pyrenoid-like aggregation for Rubisco derived from a higher plant.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.