ArticleGenome biology and evolution2019
Evolutionary Dissection of the Dot/Icm System Based on Comparative Genomics of 58 Legionella Species.
Article in Genome biology and evolution, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Article
- Multi-omics provides functional insights and underscores practical challenges in assessing the composition and performance of a nitrifying microbial consortium.Applied and environmental microbiology · 2026Article
- Transmembrane effector substrates of type IV secretion systems: mechanisms of secretion and insertion into host cell membranes.microLife · 2026Review
- Molecular evolution and adaptations ofFrontiers in cellular and infection microbiology · 2026Review
- Comparative single-cell genomics of two uncultivatedmSphere · 2025Article
- In situ structures of theProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Membrane association and polar localization of theProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Structure and Function of the Dot/Icm T4SS.bioRxiv : the preprint server for biology · 2023Article
- Functional characterization of VirB/VirD4 and Icm/Dot type IV secretion systems from the plant-pathogenic bacteriumFrontiers in cellular and infection microbiology · 2023Article
- Concept about the Virulence Factor ofMicroorganisms · 2022Review
- Host Adaptation in Legionellales Is 1.9 Ga, Coincident with Eukaryogenesis.Molecular biology and evolution · 2022Article
- Recent advances in structural studies of the Legionella pneumophila Dot/Icm type IV secretion system.Microbiology and immunology · 2022Review
- Incompatibility Group I1 (IncI1) Plasmids: Their Genetics, Biology, and Public Health Relevance.Microbiology and molecular biology reviews : MMBR · 2021Review
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Authors and funding
4 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Dot/Icm type IVB secretion system of Legionella pneumophila is essential for its pathogenesis by delivering >300 effector proteins into the host cell. However, their precise secretion mechanism and which components interact with the host cell is only partly understood. Here, we undertook evolutionary analyses of the Dot/Icm system of 58 Legionella species to identify those components that interact with the host and/or the substrates. We show that high recombination rates are acting on DotA, DotG, and IcmX, supporting exposure of these proteins to the host. Specific amino acids under positive selection on the periplasmic region of DotF, and the cytoplasmic domain of DotM, support a role of these regions in substrate binding. Diversifying selection acting on the signal peptide of DotC suggests its interaction with the host after cleavage. Positive selection acts on IcmR, IcmQ, and DotL revealing that these components are probably participating in effector recognition and/or translocation. Furthermore, our results predict the participation in host/effector interaction of DotV and IcmF. In contrast, DotB, DotO, most of the core subcomplex elements, and the chaperones IcmS-W show a high degree of conservation and not signs of recombination or positive selection suggesting that these proteins are under strong structural constraints and have an important role in maintaining the architecture/function of the system. Thus, our analyses of recombination and positive selection acting on the Dot/Icm secretion system predicted specific Dot/Icm components and regions implicated in host interaction and/or substrate recognition and translocation, which will guide further functional analyses.
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