ArticleChemical senses2019
Optimization of Insect Odorant Receptor Trafficking and Functional Expression Via Transient Transfection in HEK293 Cells.
Article in Chemical senses, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 18 citations in OpenAlex.
- An anhydrobiotic cell line expressing odorant receptors shows odorant responses after dry storage.Scientific reports · 2025Article
- Probing the optimal architecture and molecular mechanism of insect odorant receptor heteromeric channels.Communications biology · 2025Article
- Alternative strategies based on transgenic Drosophila melanogaster for the functional characterization of insect Ionotropic Receptors.Biological research · 2025Article
- Functional Characterization Supports Multiple Evolutionary Origins of Pheromone Receptors in Bark Beetles.Molecular biology and evolution · 2024Article
- High-throughput ligand profile characterization in novel cell lines expressing seven heterologous insect olfactory receptors for the detection of volatile plant biomarkers.Scientific reports · 2023Article
- Impacts of OrX and cAMP-insensitive Orco to the insect olfactory heteromer activity.Molecular biology reports · 2021Article
- Codon Optimization of Insect Odorant Receptor Genes May Increase Their Stable Expression for Functional Characterization in HEK293 Cells.Frontiers in cellular neuroscience · 2021Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
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Abstract
Insect odorant receptors (ORs) show a limited functional expression in various heterologous expression systems including insect and mammalian cells. This may be in part due to the absence of key components driving the release of these proteins from the endoplasmic reticulum and directing them to the plasma membrane. In order to mitigate this problem, we took advantage of small export signals within the human HCN1 and Rhodopsin that have been shown to promote protein release from the endoplasmic reticulum and the trafficking of post-Golgi vesicles, respectively. Moreover, we designed a new vector based on a bidirectional expression cassette to drive the functional expression of the insect odorant receptor coreceptor (Orco) and an odor-binding OR, simultaneously. We show that this new method can be used to reliably express insect ORs in HEK293 cells via transient transfection and that is highly suitable for downstream applications using automated and high-throughput imaging platforms.
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