Evidence map›Paper›PMID 31503560›Full record

ArticleJournal of the Optical Society of America. A, Optics, image science, and vision2019

Spatio-angular fluorescence microscopy II. Paraxial 4f imaging.

Talon Chandler, Hari Shroff, Rudolf Oldenbourg, Patrick La Rivière

Abstract read
In one paragraph

Article in Journal of the Optical Society of America. A, Optics, image science, and vision, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Volumetric imaging of the 3D orientation of cellular structures with a polarized fluorescence light-sheet microscope.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Spatio-angular fluorescence microscopy III. Constrained angular diffusion, polarized excitation, and high-NA imaging.Journal of the Optical Society of America. A, Optics, image science, and vision · 2020
    Article
  12. Article
  13. Article
  14. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Talon Chandler
Hari Shroff
Rudolf Oldenbourg
Patrick La Rivière

Funding

3D imaging of cells and tissues using polarized light microscopyR35GM131843 · NIGMS · MARINE BIOLOGICAL LABORATORY · PI OLDENBOURG, RUDOLF · 2019 to 2023
$2.5M
Broadband X-ray Fluorescence Emission TomographyR01EB026300 · NIBIB · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · PI LA RIVIERE, PATRICK JEAN, LU, YI · 2018 to 2021
$2.4M
Instantaneous 3D imaging of cells and tissues using polarized light microscopyR01GM114274 · NIGMS · MARINE BIOLOGICAL LABORATORY · PI OLDENBOURG, RUDOLF · 2015 to 2018
$2.4M
Development of a benchtop x-ray fluorescence tomography system using a novel geomR01EB017293 · NIBIB · UNIVERSITY OF CHICAGO · PI LA RIVIERE, PATRICK JEAN, MENG, LING-JIAN · 2014 to 2017
$1.8M
NIBIB NIH HHS R01 EB017293NIBIB NIH HHS R01 EB026300NIGMS NIH HHS R01 GM114274NIGMS NIH HHS R35 GM131843
6 · The paper itself

Abstract

We investigate the properties of a single-view fluorescence microscope in a 4f geometry when imaging fluorescent dipoles without using the monopole or scalar approximations. We show that this imaging system has a spatio-angular band limit, and we exploit the band limit to perform efficient simulations. Notably, we show that information about the out-of-plane orientation of ensembles of in-focus fluorophores is recorded by paraxial fluorescence microscopes. Additionally, we show that the monopole approximation may cause biased estimates of fluorophore concentrations, but these biases are small when the sample contains either many randomly oriented fluorophores in each resolvable volume or unconstrained rotating fluorophores.

Identifiers

PMID31503560
PMCPMC7045803

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.