ArticleThe British journal of surgery2020
Dysregulation of the actin scavenging system and inhibition of DNase activity following severe thermal injury.
Article in The British journal of surgery, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT04372979 (Evaluation Of Efficacy Of COVID-19 Convalescent Plasma Versus Standard Plasma In The Early Care Of COVID-19 Patients Hospitalized Outside Intensive Care Units.), which is not on this map. Cited by 22 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Evaluation Of Efficacy Of COVID-19 Convalescent Plasma Versus Standard Plasma In The Early Care Of COVID-19 Patients Hospitalized Outside Intensive Care Units.
Who cites it
22 citing papers in PubMed, 32 citations in OpenAlex.
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- Development of DNase-1 Loaded Polymeric Nanoparticles Synthesized by Inverse Flash Nanoprecipitation for Neutrophil-Mediated Drug Delivery to In Vitro Thrombi.Advanced healthcare materials · 2025Article
- Kinetics of DNases after hematopoietic stem cell transplant.Blood vessels, thrombosis & hemostasis · 2025Article
- Degradation rather than disassembly of necrotic debris is essential to enhance recovery after acute liver injury.Cellular and molecular life sciences : CMLS · 2025Article
- Phalloidin and DNase I-bound F-actin pointed end structures reveal principles of filament stabilization and disassembly.Nature communications · 2024Article
- Systemic mechanisms of necrotic cell debris clearance.Cell death & disease · 2024Review
- IL-8 Induces Neutrophil Extracellular Trap Formation in Severe Thermal Injury.International journal of molecular sciences · 2024Article
- Effects of cell-free DNA on kidney disease and intervention strategies.Frontiers in pharmacology · 2024Review
- Cell-Free DNA in Plasma and Serum Indicates Disease Severity and Prognosis in Blunt Trauma Patients.Diagnostics (Basel, Switzerland) · 2023Article
- Changes in Burn Wound Microbiology Profile Over 14 Years of an Adult Tertiary Burn Center.Journal of burn care & research : official publication of the American Burn Association · 2023Article
- Murine scald models characterize the role of neutrophils and neutrophil extracellular traps in severe burns.Frontiers in immunology · 2023Article
- Functional proteomic profiling links deficient DNA clearance with increased mortality in individuals with severe COVID-19 pneumonia.Immunity · 2022Article
- Adjunctive Recombinant Human Plasma Gelsolin for Severe Coronavirus Disease 2019 Pneumonia.Open forum infectious diseases · 2022Article
- Exploratory Investigation of the Plasma Proteome Associated with the Endotheliopathy of Trauma.International journal of molecular sciences · 2022Article
- The role of neutrophil extracellular traps in acute lung injury.Frontiers in immunology · 2022Review
- The Pathophysiologic Role of Gelsolin in Chronic Kidney Disease: Focus on Podocytes.International journal of molecular sciences · 2021Article
- Plasma proteomic profile associated with platelet dysfunction after trauma.Journal of thrombosis and haemostasis : JTH · 2021Observational
- The Emerging Role of Neutrophil Extracellular Traps in Arterial, Venous and Cancer-Associated Thrombosis.Frontiers in cardiovascular medicine · 2021Review
- Neutrophils and COVID-19: Active Participants and Rational Therapeutic Targets.Frontiers in immunology · 2021Review
Corrections and comments
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Authors and funding
17 authors at 5 institutions in 3 countries.
Funding
Abstract
backgroundCirculating cell-free DNA (cfDNA) is not found in healthy subjects, but is readily detected after thermal injury and may contribute to the risk of multiple organ failure. The hypothesis was that a postburn reduction in DNase protein/enzyme activity could contribute to the increase in cfDNA following thermal injury.
methodsPatients with severe burns covering at least 15 per cent of total body surface area were recruited to a prospective cohort study within 24 h of injury. Blood samples were collected from the day of injury for 12 months.
resultsAnalysis of blood samples from 64 patients revealed a significant reduction in DNase activity on days 1-28 after injury, compared with healthy controls. DNase protein levels were not affected, suggesting the presence of an enzyme inhibitor. Further analysis revealed that actin (an inhibitor of DNase) was present in serum samples from patients but not those from controls, and concentrations of the actin scavenging proteins gelsolin and vitamin D-binding protein were significantly reduced after burn injury. In a pilot study of ten military patients with polytrauma, administration of blood products resulted in an increase in DNase activity and gelsolin levels.
conclusionThe results of this study suggest a novel biological mechanism for the accumulation of cfDNA following thermal injury by which high levels of actin released by damaged tissue cause a reduction in DNase activity. Restoration of the actin scavenging system could therefore restore DNase activity, and reduce the risk of cfDNA-induced host tissue damage and thrombosis.
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