ArticleAmerican journal of botany2019
Seed freeze sensitivity and ex situ longevity of 295 species in the native Hawaiian flora.
Article in American journal of botany, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Long-Term Storage and Longevity of Orthodox Seeds: A Systematic Review.Frontiers in plant science · 2020Pooled it
- Measuring maternal line selection driven by differential survival in ex situ collections for plant conservation.Conservation biology : the journal of the Society for Conservation Biology · 2026Article
- Cryopreservation of Seeds and Seed Embryos in Orthodox, Intermediate, and Recalcitrant Seeded Species.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Thermal behaviour of lipids in short-lived seeds of Australian rainforest species.Annals of botany · 2025Article
- The Performance ofPlants (Basel, Switzerland) · 2025Article
- Optimizing the accession-level quantity of seeds to put into storage to minimize seed (gene)bank regeneration or re-collection.Conservation physiology · 2025Article
- Pollen banking is a critical need for conserving plant diversity.Nature plants · 2024Article
- Viability and integrity ofConservation physiology · 2024Article
- Seed Storage Physiology ofPlants (Basel, Switzerland) · 2023Article
- Seed Longevity-The Evolution of Knowledge and a Conceptual Framework.Plants (Basel, Switzerland) · 2023Review
- Insights into mechanisms of seed longevity in soybean: a review.Frontiers in plant science · 2023Review
- Choosing the Right Path for the Successful Storage of Seeds.Plants (Basel, Switzerland) · 2022Review
- Low availability of functional seed trait data from the tropics could negatively affect global macroecological studies, predictive models and plant conservation.Annals of botany · 2022Review
- Bryophyte Spores Tolerate High Desiccation Levels and Exposure to Cryogenic Temperatures but Contain Storage Lipids and Chlorophyll: Understanding the Essential Traits Needed for the Creation of Bryophyte Spore Banks.Plants (Basel, Switzerland) · 2022Article
- Plant Diversity Conservation Challenges and Prospects-The Perspective of Botanic Gardens and the Millennium Seed Bank.Plants (Basel, Switzerland) · 2021Review
- Seed freeze sensitivity and ex situ longevity of 295 species in the native Hawaiian flora.American journal of botany · 2019Article
- Seed dormancy and germination of the endangered exceptional Hawaiian lobelioidApplications in plant sciencesArticle
- Supporting long-term sustainability of ex situ collections using a pedigree-based population management approach.Applications in plant sciencesArticle
- Embryo excision in Compositae, with implications for combating biodiversity loss.Applications in plant sciencesArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
premiseEx situ seed banking is critical for plant conservation globally, especially for threatened floras in tropical ecosystems like Hawai'i. Seed bank managers must maximize longevity, and species managers must plan restoration before seeds lose viability. Previous observations suggested some native Hawaiian seeds lost viability in frozen storage (-18°C). We investigated seed storage behavior in the Hawaiian flora to optimize storage conditions and recommend re-collection intervals (RCI) to maximize viability of stored seeds.
methodsUsing 20+ years of real-time seed storage viability data, we tested freeze sensitivity for 197 species and calculated RCIs for 295 species. Using paired tests of accessions stored >2 yr at 5°C and -18°C, we developed an index of relative performance to determine freeze sensitivity. We calculated RCIs at 70% of highest germination (P70).
resultsWe identified four families (Campanulaceae, Cyperaceae, Rubiaceae, and Urticaceae) and four genera with seed freeze sensitivity and six additional genera with likely freeze sensitivity. Storage longevity was variable, but 195 species had viability >70% at the most recent tests (1 to 20+ yr), 123 species had RCIs >10 yr, and 45 species had RCIs <5 yr.
conclusionsFreeze sensitive storage behavior is more widely observed in Hawai'i than any other regional flora, perhaps due to insufficient testing elsewhere. We present a new protocol to test seed freeze sensitivity, which is often not evident until 2-5 years of storage. Re-collection intervals will guide restoration practices in Hawai'i, and results inform seed conservation efforts globally, especially tropical and subtropical regions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.