ArticleNature methods2019
FRET-assisted photoactivation of flavoproteins for in vivo two-photon optogenetics.
Article in Nature methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- Lights up on the embryonic dance: tools and applications of optogenetics in developmental biology.Genes & development · 2026Review
- Endogenous OptoRhoGEFs reveal biophysical principles of epithelial tissue furrowing.Nature communications · 2025Article
- Live imaging of paracrine signaling: Advances in visualization and tracking techniques.Cell structure and function · 2025Review
- Expanding the fluorescent toolkit: Blue fluorescent protein-expressing Plasmodium berghei for enhanced multiplex microscopy.PloS one · 2025Article
- Enhancement of Vivid-based photo-activatable Gal4 transcription factor in mammalian cells.Cell structure and function · 2023Article
- In vivo imaging of inflammatory response in cancer research.Inflammation and regeneration · 2023Review
- LOV2-based photoactivatable CaMKII and its application to single synapses: Local Optogenetics.Biophysics and physicobiology · 2023Article
- Design and engineering of light-sensitive protein switches.Current opinion in structural biology · 2022Review
- Engineering rhodopsins' activation spectra using a FRET-based approach.Biophysical journal · 2022Article
- Optogenetic control of the Bicoid morphogen reveals fast and slow modes of gap gene regulation.Cell reports · 2022Article
- Light-regulated gene expression in Bacteria: Fundamentals, advances, and perspectives.Frontiers in bioengineering and biotechnology · 2022Review
- Multiphoton intravital microscopy of rodents.Nature reviews. Methods primers · 2022Article
- Cell-Cell Communication Networks in Tissue: Toward Quantitatively Linking Structure with Function.Current opinion in systems biology · 2021Article
- Signaling, Deconstructed: Using Optogenetics to Dissect and Direct Information Flow in Biological Systems.Annual review of biomedical engineering · 2021Review
- Steering Molecular Activity with Optogenetics: Recent Advances and Perspectives.Advanced biology · 2021Review
- The frontier of live tissue imaging across space and time.Cell stem cell · 2021Review
- Visualization and Manipulation of Intracellular Signaling.Advances in experimental medicine and biology · 2021Review
- Reverse and Forward Engineering Multicellular Structures with Optogenetics.Current opinion in biomedical engineering · 2020Article
- Collectively stabilizing and orienting posterior migratory forces disperses cell clusters in vivo.Nature communications · 2020Article
- FRET-Based CaSensors (Basel, Switzerland) · 2020Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Optical dimerizers have been developed to untangle signaling pathways, but they are of limited use in vivo, partly due to their inefficient activation under two-photon (2P) excitation. To overcome this problem, we developed Förster resonance energy transfer (FRET)-assisted photoactivation, or FRAPA. On 2P excitation, mTagBFP2 efficiently absorbs and transfers the energy to the chromophore of CRY2. Based on structure-guided engineering, a chimeric protein with 40% FRET efficiency was developed and named 2P-activatable CRY2, or 2paCRY2. 2paCRY2 was employed to develop a RAF1 activation system named 2paRAF. In three-dimensionally cultured cells expressing 2paRAF, extracellular signal-regulated kinase (ERK) was efficiently activated by 2P excitation at single-cell resolution. Photoactivation of ERK was also accomplished in the epidermal cells of 2paRAF-expressing mice. We further developed an mTFP1-fused LOV domain that exhibits efficient response to 2P excitation. Collectively, FRAPA will pave the way to single-cell optical control of signaling pathways in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.