Evidence map›Paper›PMID 31501469›Full record

ArticleScientific reports2019

Unveiling the multi-step solubilization mechanism of sub-micron size vesicles by detergents.

Paul A Dalgarno, José Juan-Colás, Gordon J Hedley, Lucas Piñeiro, Mercedes Novo, Cibran Perez-Gonzalez, Ifor D W Samuel, Mark C Leake, Steven Johnson, Wajih Al-Soufi and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 27 citations in OpenAlex.

  1. Article
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  5. Tween-20 Induces the Structural Remodeling of Single Lipid Vesicles.The journal of physical chemistry letters · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 2 countries.

Paul A DalgarnoSUPA School of Physics and Astronomy, University of St. Andrews, North Haugh, Fife, KY16 9SS, UK.
José Juan-ColásDepartment of Electronic Engineering, University of York, Heslington, York, YO10 5DD, UK.
Gordon J HedleySUPA School of Physics and Astronomy, University of St. Andrews, North Haugh, Fife, KY16 9SS, UK.ORCID http://orcid.org/0000-0002-1825-6296
Lucas PiñeiroDepartment of Physical Chemistry, Faculty of Science, University of Santiago de Compostela, Lugo, E-27002, Spain.
Mercedes NovoDepartment of Physical Chemistry, Faculty of Science, University of Santiago de Compostela, Lugo, E-27002, Spain.ORCID http://orcid.org/0000-0002-4869-289X
Cibran Perez-GonzalezSUPA School of Physics and Astronomy, University of St. Andrews, North Haugh, Fife, KY16 9SS, UK.
Ifor D W SamuelSUPA School of Physics and Astronomy, University of St. Andrews, North Haugh, Fife, KY16 9SS, UK.ORCID http://orcid.org/0000-0001-7821-7208
Mark C LeakeDepartment of Physics, University of York, Heslington, York, England, YO10 5DD, UK.
Steven JohnsonDepartment of Electronic Engineering, University of York, Heslington, York, YO10 5DD, UK.
Wajih Al-SoufiDepartment of Physical Chemistry, Faculty of Science, University of Santiago de Compostela, Lugo, E-27002, Spain.
J Carlos PenedoSUPA School of Physics and Astronomy, University of St. Andrews, North Haugh, Fife, KY16 9SS, UK. jcp10@st-andrews.ac.uk.
Steven D QuinnSUPA School of Physics and Astronomy, University of St. Andrews, North Haugh, Fife, KY16 9SS, UK. steven.quinn@york.ac.uk.ORCID http://orcid.org/0000-0003-3442-4103
University of St Andrews · GBUniversidade de Santiago de Compostela · ESUniversity of York · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The solubilization of membranes by detergents is critical for many technological applications and has become widely used in biochemistry research to induce cell rupture, extract cell constituents, and to purify, reconstitute and crystallize membrane proteins. The thermodynamic details of solubilization have been extensively investigated, but the kinetic aspects remain poorly understood. Here we used a combination of single-vesicle Förster resonance energy transfer (svFRET), fluorescence correlation spectroscopy and quartz-crystal microbalance with dissipation monitoring to access the real-time kinetics and elementary solubilization steps of sub-micron sized vesicles, which are inaccessible by conventional diffraction-limited optical methods. Real-time injection of a non-ionic detergent, Triton X, induced biphasic solubilization kinetics of surface-immobilized vesicles labelled with the Dil/DiD FRET pair. The nanoscale sensitivity accessible by svFRET allowed us to unambiguously assign each kinetic step to distortions of the vesicle structure comprising an initial fast vesicle-swelling event followed by slow lipid loss and micellization. We expect the svFRET platform to be applicable beyond the sub-micron sizes studied here and become a unique tool to unravel the complex kinetics of detergent-lipid interactions.

Identifiers

PMID31501469
PMCPMC6733941
OpenAlexW2972523207

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.