ArticleProceedings of the National Academy of Sciences of the United States of America2019
Digital-resolution detection of microRNA with single-base selectivity by photonic resonator absorption microscopy.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 78 citations in OpenAlex.
- PRICE: direct and robust detection of microRNAs at single-nucleotide resolution.Nature communications · 2026Article
- Kinetic Profiling in One-Step Digital Immunoassays Enables Multiplex Quantification across an Ultrabroad Dynamic Range.Journal of the American Chemical Society · 2026Article
- Ultrasensitive non-enzymatic protein detection using proximity immunoassay with photonic resonator absorption microscopy.Npj biosensing · 2026Article
- Photonic resonator absorption microscopy: why consider metallic and magneto-plasmonic nano-assemblies over bare nanoparticles for digital biosensing?Analytical and bioanalytical chemistry · 2025Article
- Oligonucleotide surface attachment by tosylation for digital detection of microRNA using photonic resonator absorption microscopy.APL bioengineering · 2025Article
- Co-allosteric hairpin probe for detecting microRNA with high specificity.RSC advances · 2025Article
- Physically grounded deep learning-enabled gold nanoparticle localization and quantification in photonic resonator absorption microscopy for digital resolution molecular diagnostics.Biosensors & bioelectronics · 2025Article
- Digital Immunoassay for Rapid Detection of SARS-CoV-2 exposure in a Broad Spectrum of Animals.IEEE sensors journal · 2025Article
- Photonic Crystal Enhanced Microscopy on a 2D Photonic Crystal Surface.Advanced materials technologies · 2025Article
- Bioinspired designer DNA NanoGripper for virus sensing and potential inhibition.Science robotics · 2024Article
- Dynamic and large field of view photonic resonator absorption microscopy for ultrasensitive digital resolution detection of nucleic acid and protein biomarkers.Biosensors & bioelectronics · 2024Article
- Portable, smartphone-linked, and miniaturized photonic resonator absorption microscope (PRAM Mini) for point-of-care diagnostics.Biomedical optics express · 2024Article
- Review of Gold Nanoparticles in Surface Plasmon-Coupled Emission Technology: Effect of Shape, Hollow Nanostructures, Nano-Assembly, Metal-Dielectric and Heterometallic Nanohybrids.Nanomaterials (Basel, Switzerland) · 2024Review
- Biomarkers for immunotherapy of hepatocellular carcinoma.Nature reviews. Clinical oncology · 2023Review
- Digital and Absolute Assays for Low Abundance Molecular Biomarkers.Accounts of chemical research · 2023Article
- A Target Recycling Amplification Process for the Digital Detection of Exosomal MicroRNAs through Photonic Resonator Absorption Microscopy.Angewandte Chemie (International ed. in English) · 2023Article
- Photonic Crystal Enhanced Fluorescence: A Review on Design Strategies and Applications.Micromachines · 2023Review
- Review of HIV Self Testing Technologies and Promising Approaches for the Next Generation.Biosensors · 2023Review
- Attomolar sensitivity microRNA detection using real-time digital microarrays.Scientific reports · 2022Article
- Label-free optical biosensors in the pandemic era.Nanophotonics (Berlin, Germany) · 2022Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Circulating exosomal microRNA (miR) represents a new class of blood-based biomarkers for cancer liquid biopsy. The detection of miR at a very low concentration and with single-base discrimination without the need for sophisticated equipment, large volumes, or elaborate sample processing is a challenge. To address this, we present an approach that is highly specific for a target miR sequence and has the ability to provide "digital" resolution of individual target molecules with high signal-to-noise ratio. Gold nanoparticle tags are prepared with thermodynamically optimized nucleic acid toehold probes that, when binding to a target miR sequence, displace a probe-protecting oligonucleotide and reveal a capture sequence that is used to selectively pull down the target-probe-nanoparticle complex to a photonic crystal (PC) biosensor surface. By matching the surface plasmon-resonant wavelength of the nanoparticle tag to the resonant wavelength of the PC nanostructure, the reflected light intensity from the PC is dramatically and locally quenched by the presence of each individual nanoparticle, enabling a form of biosensor microscopy that we call Photonic Resonator Absorption Microscopy (PRAM). Dynamic PRAM imaging of nanoparticle tag capture enables direct 100-aM limit of detection and single-base mismatch selectivity in a 2-h kinetic discrimination assay. The PRAM assay demonstrates that ultrasensitivity (<1 pM) and high selectivity can be achieved on a direct readout diagnostic.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.