Evidence map›Paper›PMID 31500728›Full record

ArticleVeterinary microbiology2019

Peptides with 16R in S2 protein showed broad reactions with sera against different types of infectious bronchitis viruses.

Qi Wu, Zhixian Lin, Kun Qian, Hongxia Shao, Jianqiang Ye, Aijian Qin

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.1field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Qi WuKey Laboratory of Avian Preventive Medicine, Ministry of Education, Yangzhou University, China; The International Joint Laboratory for Cooperation in Agriculture and Agricultural Product Safety, Ministry of Education, Yangzhou University, China. Electronic address: 1713497665@qq.com.
Zhixian LinKey Laboratory of Avian Preventive Medicine, Ministry of Education, Yangzhou University, China; The International Joint Laboratory for Cooperation in Agriculture and Agricultural Product Safety, Ministry of Education, Yangzhou University, China. Electronic address: 29811883878@qq.com.
Kun QianKey Laboratory of Avian Preventive Medicine, Ministry of Education, Yangzhou University, China; The International Joint Laboratory for Cooperation in Agriculture and Agricultural Product Safety, Ministry of Education, Yangzhou University, China; Jiangsu Key Laboratory of Preventive Veterinary Medicine, Yangzhou University, China. Electronic address: qiankun@yzu.edu.cn.
Hongxia ShaoKey Laboratory of Avian Preventive Medicine, Ministry of Education, Yangzhou University, China; The International Joint Laboratory for Cooperation in Agriculture and Agricultural Product Safety, Ministry of Education, Yangzhou University, China; Jiangsu Key Laboratory of Preventive Veterinary Medicine, Yangzhou University, China. Electronic address: 987137092@qq.com.
Jianqiang YeKey Laboratory of Avian Preventive Medicine, Ministry of Education, Yangzhou University, China; The International Joint Laboratory for Cooperation in Agriculture and Agricultural Product Safety, Ministry of Education, Yangzhou University, China; Jiangsu Key Laboratory of Preventive Veterinary Medicine, Yangzhou University, China. Electronic address: jqye@yzu.edu.cn.
Aijian QinKey Laboratory of Avian Preventive Medicine, Ministry of Education, Yangzhou University, China; The International Joint Laboratory for Cooperation in Agriculture and Agricultural Product Safety, Ministry of Education, Yangzhou University, China; Jiangsu Key Laboratory of Preventive Veterinary Medicine, Yangzhou University, China. Electronic address: aijian@yzu.edu.cn.
Yangzhou University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Vaccination plays a vital role in controlling diseases caused by chicken infectious bronchitis virus (IBV). The continuously variant antigenicity of IBV limits the application of current vaccine strategies and serological diagnostic systems. S2 protein is an invariant that harbors broad neutralizing epitopes. However, little is known about the key amino acids that contribute to the broad-spectrum S2 epitopes. In this study, we aimed to elucidate the specific amino acids contributing to S2 epitopes. Site mutagenesis and peptide-based enzyme-linked immunosorbent assays (ELISAs) showed that 16R in S2 protein was a key amino acid mediating the antigenicity of S2 protein. S2-derived peptides with 16R, but not those with 16 K, could react with sera against different types of IBVs. Notably, a commercial ELISA kit for detection of antibodies against IBV did not react with sera against all types of IBVs. Taken together, these data demonstrated that S2-derived peptides with 16R could be used as novel marker-based antigens for developing both broad-spectrum vaccines and serological diagnostic kits to control IBV.

Indexed as

Amino Acid SequenceAnimalsEpitopesGenotypeImmune SeraInfectious bronchitis virusViral ProteinsEpitopesImmune SeraViral Proteins16RInfectious bronchitis virusPeptideS2 proteinSerology

Identifiers

PMID31500728
PMCPMC7117385
OpenAlexW2968174590

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.