Evidence map›Paper›PMID 31500151›Full record

ArticleCells2019

Viscosity of Plasma as a Key Factor in Assessment of Extracellular Vesicles by Light Scattering.

Darja Božič, Simona Sitar, Ita Junkar, Roman Štukelj, Manca Pajnič, Ema Žagar, Veronika Kralj-Iglič, Ksenija Kogej

Abstract read
In one paragraph

Article in Cells, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Darja BožičDepartment of Chemistry and Biochemistry, University of Ljubljana, Faculty of Chemistry and Chemical Technology, SI-1000 Ljubljana, Slovenia.
Simona SitarDepartment of Polymer Chemistry and Technology, National Institute of Chemistry, SI-1001 Ljubljana, Slovenia.
Ita JunkarDepartment of Surface Engineering and Optoelectronics, Josef Stefan Institute, SI-1000 Ljubljana, Slovenia.
Roman ŠtukeljBiomedical Research Group, University of Ljubljana, Faculty of Health Sciences, SI-1000 Ljubljana, Slovenia.
Manca PajničBiomedical Research Group, University of Ljubljana, Faculty of Health Sciences, SI-1000 Ljubljana, Slovenia.
Ema ŽagarDepartment of Polymer Chemistry and Technology, National Institute of Chemistry, SI-1001 Ljubljana, Slovenia.
Veronika Kralj-IgličBiomedical Research Group, University of Ljubljana, Faculty of Health Sciences, SI-1000 Ljubljana, Slovenia.
Ksenija KogejDepartment of Chemistry and Biochemistry, University of Ljubljana, Faculty of Chemistry and Chemical Technology, SI-1000 Ljubljana, Slovenia. ksenija.kogej@fkkt.uni-lj.si.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Extracellular vesicles (EVs) isolated from biological samples are a promising material for use in medicine and technology. However, the assessment methods that would yield repeatable concentrations, sizes and compositions of the harvested material are missing. A plausible model for the description of EV isolates has not been developed. Furthermore, the identity and genesis of EVs are still obscure and the relevant parameters have not yet been identified. The purpose of this work is to better understand the mechanisms taking place during harvesting of EVs, in particular the role of viscosity of EV suspension. The EVs were harvested from blood plasma by repeated centrifugation and washing of samples. Their size and shape were assessed by using a combination of static and dynamic light scattering. The average shape parameter of the assessed particles was found to be

Indexed as

AdultBlood ViscosityCentrifugationDynamic Light ScatteringExosomesExtracellular VesiclesFemaleHealthy VolunteersHumansMaleMiddle AgedPlasmaSpecimen HandlingViscosityblood plasmadynamic light scatteringexosomesextracellular vesiclesshape characterizationstatic light scatteringviscosity of blood plasma

Identifiers

PMID31500151
PMCPMC6769602

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.