ArticleBiomacromolecules2019
Extracellular Antifreeze Protein Significantly Enhances the Cryopreservation of Cell Monolayers.
Article in Biomacromolecules, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Rational engineering of an antifreeze-based biomaterial with dual-enhanced ice-binding and delivery for high-efficiency cryopreservation.Journal of advanced research · 2026Article
- Antifreezing hydrogels for biomedical applications from design strategies to emerging multifunctionality.Bioactive materials · 2026Review
- Cryopreservation of Avian Erythrocytes Using Macromolecular Cryoprotectants.ACS polymers Au · 2026Article
- Ice-binding protein's dual roles for prolonging the enzymatic activity of alcohol dehydrogenase against freezing.International journal of biological macromolecules · 2026Article
- Antarctic microbial protein-rich extracts with cryoprotective potential for cell and viral preservation.Frontiers in microbiology · 2026Article
- Research progress on novel bionic cryoprotectants in gamete and embryo cryopreservation.Frontiers in veterinary science · 2026Review
- Antifreeze proteins produced by Antarctic yeast from the genus Glaciozyma as cryoprotectants in food storage.PloS one · 2025Article
- The protective effect of zinc oxide and selenium oxide nanoparticles on the functional parameters of rat sperm during vitrification.Clinical and experimental reproductive medicine · 2024Article
- Cryopreservation of assay-ready hepatocyte monolayers by chemically-induced ice nucleation: preservation of hepatic function and hepatotoxicity screening capabilities.Biomaterials science · 2023Article
- High Molecular Weight Polyproline as a Potential Biosourced Ice Growth Inhibitor: Synthesis, Ice Recrystallization Inhibition, and Specific Ice Face Binding.Biomacromolecules · 2023Article
- The effects of purslane (Portulaca oleracea) and fennel (Foeniculum vulgare Mill) hydroalcoholic extracts on the functional parameters of human spermatozoa after vitrification.Clinical and experimental reproductive medicine · 2023Article
- Article
- Microcurvature Controllable Metal-Organic Framework Nanoagents Capable of Ice-Lattice Matching for Cellular Cryopreservation.JACS Au · 2023Article
- Incorporate delivery, warming and washing methods into efficient cryopreservation.Frontiers in bioengineering and biotechnology · 2023Article
- Assay-ready Cryopreserved Cell Monolayers Enabled by Macromolecular Cryoprotectants.Biomacromolecules · 2022Article
- Pollen derived macromolecules serve as a new class of ice-nucleating cryoprotectants.Scientific reports · 2022Article
- Degradable Polyampholytes from Radical Ring-Opening Copolymerization Enhance Cellular Cryopreservation.ACS macro letters · 2022Article
- Intracellular and Extracellular Antifreeze Protein Significantly Improves Mammalian Cell Cryopreservation.Biomolecules · 2022Article
- Antifreeze Proteins: Novel Applications and Navigation towards Their Clinical Application in Cryobanking.International journal of molecular sciences · 2022Review
- An insect antifreeze protein from Anatolica polita enhances the cryoprotection of Xenopus laevis eggs and embryos.The Journal of experimental biology · 2022Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The cryopreservation of cells underpins many areas of biotechnology, healthcare, and fundamental science by enabling the banking and distribution of cells. Cryoprotectants are essential to prevent cold-induced damage. Here, we demonstrate that extracellular localization of antifreeze proteins can significantly enhance post-thaw recovery of mammalian cell monolayers cryopreserved using dimethyl sulfoxide, whereas they show less benefit in suspension cryopreservation. A type III antifreeze protein (AFPIII) was used as the macromolecular ice recrystallization inhibitor and its intra/extracellular locations were controlled by using Pep-1, a cell-penetrating peptide. Flow cytometry and confocal microscopy confirmed successful delivery of AFPIII. The presence of extracellular AFPIII dramatically increased post-thaw recovery in a challenging 2-D cell monolayer system using just 0.8 mg·mL
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.