ArticleJournal of visualized experiments : JoVE2019
Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals.
Article in Journal of visualized experiments : JoVE, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed.
- Illuminating native fluorescence signatures for cancer detection.Proceedings of SPIE--the International Society for Optical Engineering · 2026Article
- Combined excitation scan-based hyperspectral imaging and digital deconvolution for the assessment of the kinetic and spatial distributions of cAMP signals.Proceedings of SPIE--the International Society for Optical Engineering · 2026Article
- Design of Multiplexed, Live Cell Imaging Experiments Using Excitation Scan-Based Hyperspectral Imaging Microscopy.Proceedings of SPIE--the International Society for Optical Engineering · 2025Article
- A naturally brighter approach to colorectal cancer detection.Proceedings of SPIE--the International Society for Optical Engineering · 2025Article
- Hyperspectral imaging and dynamic region of interest tracking approaches to quantify localized cAMP signals.Biochemical Society transactions · 2024Review
- Comparing Performance of Spectral Image Analysis Approaches for Detection of Cellular Signals in Time-Lapse Hyperspectral Imaging Fluorescence Excitation-Scanning Microscopy.Bioengineering (Basel, Switzerland) · 2023Article
- Multifaceted mirror array illuminator for fluorescence excitation-scanning spectral imaging microscopy.Journal of biomedical optics · 2023Article
- Live-cell fluorescence spectral imaging as a data science challenge.Biophysical reviews · 2022Review
- Application of Microspectral Imaging in Motor and Sensory Nerve Classification.Journal of healthcare engineering · 2021Article
- Comparison of spectral FRET microscopy approaches for single-cell analysis.Proceedings of SPIE--the International Society for Optical Engineering · 2020Article
- Combined hyperspectral imaging, monolayer stress microscopy, and S8 image analysis approaches for simultaneously interrogating cellular signals and biomechanics.Proceedings of SPIE--the International Society for Optical EngineeringArticle
- Validation of Excitation-Scan Hyperspectral Multi-faceted Mirror Array Prototype System Advancements to Hyperspectral Imaging Applications.Proceedings of SPIE--the International Society for Optical EngineeringArticle
- High-speed fluorescence excitation-scanning hyperspectral imaging microscopy using thin-film tunable filters.Proceedings of SPIE--the International Society for Optical EngineeringArticle
- Measurement of agonist-induced CaProceedings of SPIE--the International Society for Optical EngineeringArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Several techniques rely on detection of fluorescence signals to identify or study phenomena or to elucidate functions. Separation of these fluorescence signals were proven cumbersome until the advent of hyperspectral imaging, in which fluorescence sources can be separated from each other as well as from background signals and autofluorescence (given knowledge of their spectral signatures). However, traditional, emission-scanning hyperspectral imaging suffers from slow acquisition times and low signal-to-noise ratios due to the necessary filtering of both excitation and emission light. It has been previously shown that excitation-scanning hyperspectral imaging reduces the necessary acquisition time while simultaneously increasing the signal-to-noise ratio of acquired data. Using commercially available equipment, this protocol describes how to assemble, calibrate, and use an excitation-scanning hyperspectral imaging microscopy system for separation of signals from several fluorescence sources in a single sample. While highly applicable to microscopic imaging of cells and tissues, this technique may also be useful for any type of experiment utilizing fluorescence in which it is possible to vary excitation wavelengths, including but not limited to: chemical imaging, environmental applications, eye care, food science, forensic science, medical science, and mineralogy.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.