ArticlePhilosophical transactions of the Royal Society of London. Series B, Biological sciences2019
The expression and characterization of recombinant cp19k barnacle cement protein from Pollicipes pollicipes.
Article in Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 29 citations in OpenAlex.
- Bioinspired bioadhesion: translating nature's adhesive strategies into regenerative medicine.Med-X · 2026Review
- Recombinant Proteins: A Molecular Tool to Understand Marine Adhesion and to Advance Biomaterials.Advanced healthcare materials · 2026Review
- Amyloid-like Structures in Marine Adhesive Proteins.Marine drugs · 2025Review
- Recent Advances in Barnacle-Inspired Biomaterials in the Field of Biomedical Research.Materials (Basel, Switzerland) · 2025Review
- Self-assembly and adhesive properties ofBeilstein journal of nanotechnology · 2025Article
- An Overview on the Adhesion Mechanisms of Typical Aquatic Organisms and the Applications of Biomimetic Adhesives in Aquatic Environments.International journal of molecular sciences · 2024Review
- Self-assembly of a barnacle cement protein into intertwined amyloid fibres and determination of their adhesive and viscoelastic properties.Journal of the Royal Society, Interface · 2023Article
- High Performance Marine and Terrestrial Bioadhesives and the Biomedical Applications They Have Inspired.Molecules (Basel, Switzerland) · 2022Review
- Adhesive Materials Inspired by Barnacle Underwater Adhesion: Biological Principles and Biomimetic Designs.Frontiers in bioengineering and biotechnology · 2022Review
- An essential role of disulfide bonds for the hierarchical self-assembly and underwater affinity of CP20-derived peptides.Frontiers in bioengineering and biotechnology · 2022Article
- Disentangling the Roles of Functional Domains in the Aggregation and Adsorption of the Multimodular Sea Star Adhesive Protein Sfp1.Marine biotechnology (New York, N.Y.) · 2021Article
- Transdisciplinary approaches to the study of adhesion and adhesives in biological systems.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adhesive proteins of barnacle cement have potential as environmentally friendly adhesives owing to their ability to adhere to various substrates in aqueous environments. By understanding the taxonomic breath of barnacles with different lifestyles, we may uncover commonalities in adhesives produced by these specialized organisms. The 19 kDa cement protein (cp19k) of the stalked barnacle Pollicipes pollicipes was expressed in Escherichia coli BL21 to investigate its adhesive properties. Initial expression of hexahistidine-tagged protein (rPpolcp19k-his) yielded low levels of insoluble protein. Co-overproduction of E. coli molecular chaperones GroEL-GroES and trigger factor (TF) increased soluble protein yields, although TF co-purified with the target protein (TF-rPpolcp19k-his). Surface coat analysis revealed high levels of adsorption of the TF-rPpolcp19k-his complex and of purified E. coli TF on both hydrophobic and hydrophilic surfaces, while low levels of adsorption were observed for rPpolcp19k-his. Tag-free rPpolcp19k protein also exhibited low adsorption compared to fibrinogen and Cell-Tak controls on hydrophobic, neutral hydrophilic and charged self-assembled monolayers under surface plasmon resonance assay conditions designed to mimic the barnacle cement gland or seawater. Because rPpolcp19k protein displays low adhesive capability, this protein is suggested to confer the ability to self-assemble into a plaque within the barnacle cement complex. This article is part of the theme issue 'Transdisciplinary approaches to the study of adhesion and adhesives in biological systems'.
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