ArticleEuropean journal of epidemiology2019
Validated inference of smoking habits from blood with a finite DNA methylation marker set.
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Article in European journal of epidemiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 2 of them syntheses that pooled it.
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33citing papers in PubMed, 2 pooled it
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Who cites it
33 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Paediatric DNA methylation profile scores: a systematic review and open-source atlas.EBioMedicine · 2026Pooled it
- Pulmonary Function and Blood DNA Methylation: A Multiancestry Epigenome-Wide Association Meta-analysis.American journal of respiratory and critical care medicine · 2022Pooled it
- Epigenetic signatures in antidepressant treatment response: a methylome-wide association study in the EMC trial.Translational psychiatry · 2022Trial
- Epigenetic fingerprints link early-onset colon and rectal cancer to pesticide exposure.Nature medicine · 2026Article
- Article
- Forensic genetics in the omics era.Nature reviews. Genetics · 2026Review
- EpiSmokEr2: a robust epigenetic classifier for smoking status inference using Illumina EPIC methylation data.Epigenomics · 2026Article
- Proteomic signatures of smoking and their associations with risk of incident diseases and mortality in diverse populations.Nature communications · 2025Article
- Pre-existing DNA methylation signatures in the prefrontal cortex of alcohol-naïve nonhuman primates define neural vulnerability for future risky ethanol consumption.Neurobiology of disease · 2025Article
- Associations of DNA Methylation Algorithms of Aging With Cardiovascular Disease and Mortality Risk Among US Older Adults.Journal of the American Heart Association · 2025Article
- Investigating Single-Molecule Molecular Inversion Probes for Medium-Scale Targeted DNA Methylation Analysis.Epigenomes · 2025Article
- Emerging PFAS contaminants PFNA and PFSA amplify epigenetic aging: sex- and age-stratified risks in an aging population.Frontiers in aging · 2025Article
- Association of marital status with DNA methylation-based biological age among US older adults.Frontiers in public health · 2025Article
- DNA methylation at AHRR as a master predictor of smoke exposure and a biomarker for sleep and exercise.Clinical epigenetics · 2024Article
- Phenotype prediction using biologically interpretable neural networks on multi-cohort multi-omics data.NPJ systems biology and applications · 2024Article
- Uncovering Forensic Evidence: A Path to Age Estimation through DNA Methylation.International journal of molecular sciences · 2024Review
- Linking Prenatal Environmental Exposures to Lifetime Health with Epigenome-Wide Association Studies: State-of-the-Science Review and Future Recommendations.Environmental health perspectives · 2023Review
- Epigenetic biomarkers for smoking cessation.Addiction neuroscience · 2023Article
- Characterisation of ethnic differences in DNA methylation between UK-resident South Asians and Europeans.Clinical epigenetics · 2022Article
- Derivation and validation of an epigenetic frailty risk score in population-based cohorts of older adults.Nature communications · 2022Article
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Authors and funding
29 authors.
Silvana C E MaasDepartment of Epidemiology, Erasmus MC University Medical Center Rotterdam, Dr. Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands.
Athina VidakiDepartment of Genetic Identification, Erasmus MC University Medical Center Rotterdam, Dr. Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands.
Rory WilsonResearch Unit of Molecular Epidemiology, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Ingolstädter Landstr. 1, 85764, Neuherberg, Germany.
Alexander TeumerInstitute for Community Medicine, University Medicine Greifswald, Walther-Rathenau-Str. 48, 17475, Greifswald, Germany.
Fan LiuDepartment of Genetic Identification, Erasmus MC University Medical Center Rotterdam, Dr. Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands.
Joyce B J van MeursDepartment of Epidemiology, Erasmus MC University Medical Center Rotterdam, Dr. Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands.
André G UitterlindenDepartment of Epidemiology, Erasmus MC University Medical Center Rotterdam, Dr. Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands.
Dorret I BoomsmaDepartment of Biological Psychology, Vrije Universiteit, Van der Boechorststraat 7-9, 1081 BT, Amsterdam, The Netherlands.
Eco J C de GeusDepartment of Biological Psychology, Vrije Universiteit, Van der Boechorststraat 7-9, 1081 BT, Amsterdam, The Netherlands.
Gonneke WillemsenDepartment of Biological Psychology, Vrije Universiteit, Van der Boechorststraat 7-9, 1081 BT, Amsterdam, The Netherlands.
Jenny van DongenDepartment of Biological Psychology, Vrije Universiteit, Van der Boechorststraat 7-9, 1081 BT, Amsterdam, The Netherlands.
Carla J H van der KallenDepartment of Internal Medicine, Maastricht University Medical Center, Randwycksingel 35, 6229 EG, Maastricht, The Netherlands.
P Eline SlagboomMolecular Epidemiology, Department of Biomedical Data Sciences, Leiden University Medical Center, P.O. box 9600, 2300 RC, Leiden, The Netherlands.
Marian BeekmanMolecular Epidemiology, Department of Biomedical Data Sciences, Leiden University Medical Center, P.O. box 9600, 2300 RC, Leiden, The Netherlands.
Diana van HeemstGerontology and Geriatrics, Department of Internal Medicine, Leiden University Medical Center, P.O. box 9600, 2300 RC, Leiden, The Netherlands.
Leonard H van den BergDepartment of Neurology, Brain Center Rudolf Magnus, University Medical Center Utrecht, Postbus 85500, 3508 GA, Utrecht, The Netherlands.
BIOS Consortium
Liesbeth DuijtsDivision of Respiratory Medicine and Allergology and Division of Neonatology, Department of Pediatrics, Erasmus MC University Medical Center Rotterdam, Dr. Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands.
Vincent W V JaddoeDepartment of Epidemiology, Erasmus MC University Medical Center Rotterdam, Dr. Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands.
Karl-Heinz LadwigInstitute of Epidemiology, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Ingolstädter Landstr. 1, 85764, Neuherberg, Germany.
Sonja KunzeResearch Unit of Molecular Epidemiology, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Ingolstädter Landstr. 1, 85764, Neuherberg, Germany.
Annette PetersResearch Unit of Molecular Epidemiology, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Ingolstädter Landstr. 1, 85764, Neuherberg, Germany.
M Arfan IkramDepartment of Epidemiology, Erasmus MC University Medical Center Rotterdam, Dr. Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands.
Hans J GrabeDepartment of Psychiatry and Psychotherapy, University Medicine Greifswald, Ellernholzstraße 1-2, 17475, Greifswald, Germany.
Janine F FelixDepartment of Epidemiology, Erasmus MC University Medical Center Rotterdam, Dr. Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands.
Melanie WaldenbergerResearch Unit of Molecular Epidemiology, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Ingolstädter Landstr. 1, 85764, Neuherberg, Germany.
Oscar H FrancoDepartment of Epidemiology, Erasmus MC University Medical Center Rotterdam, Dr. Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands.
Mohsen GhanbariDepartment of Epidemiology, Erasmus MC University Medical Center Rotterdam, Dr. Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands. m.ghanbari@erasmusm.nl.
Manfred KayserDepartment of Genetic Identification, Erasmus MC University Medical Center Rotterdam, Dr. Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands. m.kayser@erasmusmc.nl.
Funding
Epigenetic Pathways to Conduct Problem Trajectories: Early Environmental RisksR01HD068437 · NICHD · KING'S COLLEGE LONDON · PI BARKER, EDWARD D., MILL, JONATHAN · 2012 to 2014
$1.1MBundesministerium für Bildung und Forschung 01ZZ0103Bundesministerium für Bildung und Forschung 01ZZ0403Bundesministerium für Bildung und Forschung 01ZZ9603Bundesministerium für Bildung und Forschung 03IS2061ADeutsches Zentrum für Herz- Kreislaufforschung 81X3400104Dutch Diabetes Research Foundation 98.901EPA EP-C-15-001FP7 Science in Society 602736FP7 Science in Society 603288H2020 European Research Council ERC-2014-CoG-648916Horizon 2020 Framework Programme 633595Horizon 2020 Framework Programme 696295Horizon 2020 Framework Programme 733206National Institute of Child Health and Human Development R01HD068437Nederlandse Organisatie voor Wetenschappelijk Onderzoek 016.136.361Nederlandse Organisatie voor Wetenschappelijk Onderzoek 050-060-810Nederlandse Organisatie voor Wetenschappelijk Onderzoek 184021007Nederlandse Organisatie voor Wetenschappelijk Onderzoek 529051014Nederlandse Organisatie voor Wetenschappelijk Onderzoek NWO 184.021.007Nederlandse Organisatie voor Wetenschappelijk Onderzoek NWO-Groot 480-15-001/674Netherlands Organization for Scientific Research 940-35-034NICHD NIH HHS R01 HD068437ZonMw 400-05-717ZonMw 451-04-034ZonMw 463-06-001ZonMw 480-04-004ZonMw 904-61-090ZonMw 904-61-193ZonMw 912-10-020ZonMw 985-10-002
Abstract
Inferring a person's smoking habit and history from blood is relevant for complementing or replacing self-reports in epidemiological and public health research, and for forensic applications. However, a finite DNA methylation marker set and a validated statistical model based on a large dataset are not yet available. Employing 14 epigenome-wide association studies for marker discovery, and using data from six population-based cohorts (N = 3764) for model building, we identified 13 CpGs most suitable for inferring smoking versus non-smoking status from blood with a cumulative Area Under the Curve (AUC) of 0.901. Internal fivefold cross-validation yielded an average AUC of 0.897 ± 0.137, while external model validation in an independent population-based cohort (N = 1608) achieved an AUC of 0.911. These 13 CpGs also provided accurate inference of current (average AUC
Indexed as
DNA MethylationAdultArea Under CurveBiomarkersCotinineDNAEpigenomicsFemaleHumansMaleReproducibility of ResultsSensitivity and SpecificitySmokingSmoking CessationBiomarkersCotinineDNADNA methylationEpidemiologyEpigeneticsForensicsSmoking inference
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