ArticleGenomics, proteomics & bioinformatics2019
Proteomics Analysis of Lipid Droplets from the Oleaginous Alga Chromochloris zofingiensis Reveals Novel Proteins for Lipid Metabolism.
Article in Genomics, proteomics & bioinformatics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 68 citations in OpenAlex.
- Chromosome-level genome assembly and DNA methylation data of the oleaginous astaxanthin-producing alga Chromochloris zofingiensis.Scientific data · 2026Article
- Horizontal gene transfer and diploidy illuminate evolution and stress adaptation in oleaginous Scenedesmaceae (Chlorophyta).Nature communications · 2026Article
- Regulatory network rewiring drives strain-specific lipid accumulation response in Chlorella sorokiniana under nutrient starvation.The Plant journal : for cell and molecular biology · 2026Article
- Astaxanthin fromMarine drugs · 2025Review
- Nonlinear relationship between lumbar bone mineral density and high-density lipoprotein cholesterol in patients with type 2 diabetes.Frontiers in endocrinology · 2025Article
- α/β hydrolase domain-containing protein 1 acts as a lysolipid lipase and is involved in lipid droplet formation.National science review · 2024Article
- Iron rescues glucose-mediated photosynthesis repression during lipid accumulation in the green alga Chromochloris zofingiensis.Nature communications · 2024Article
- Implicating the red body of Nannochloropsis in forming the recalcitrant cell wall polymer algaenan.Nature communications · 2024Article
- Lipid Droplets in EndosymbioticPlants (Basel, Switzerland) · 2024Review
- Microalgae biofuels: illuminating the path to a sustainable future amidst challenges and opportunities.Biotechnology for biofuels and bioproducts · 2024Review
- Progress towards a targeted biorefinery ofBiomass conversion and biorefinery · 2024Review
- Characterization of Auxenochlorella protothecoides acyltransferases and potential of their protein interactions to promote the enrichment of oleic acid.Biotechnology for biofuels and bioproducts · 2023Article
- Caleosin/peroxygenases: multifunctional proteins in plants.Annals of botany · 2023Review
- Bioinformatics-Based Screening Approach for the Identification and Characterization of Lipolytic Enzymes from the Marine DiatomMarine drugs · 2023Article
- Algal glycobiotechnology: omics approaches for strain improvement.Microbial cell factories · 2021Review
- The oleaginous astaxanthin-producing alga Chromochloris zofingiensis: potential from production to an emerging model for studying lipid metabolism and carotenogenesis.Biotechnology for biofuels · 2021Review
- Multi-Omics Approaches and Radiation on Lipid Metabolism in Toothed Whales.Life (Basel, Switzerland) · 2021Review
- High-Fat Diets with Differential Fatty Acids Induce Obesity and Perturb Gut Microbiota in Honey Bee.International journal of molecular sciences · 2021Article
- Astaxanthin Is Ketolated from Zeaxanthin Independent of Fatty Acid Synthesis inPlant physiology · 2020Article
- Identification of Low-Abundance Lipid Droplet Proteins in Seeds and Seedlings.Plant physiology · 2020Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chromochloris zofingiensis represents an industrially relevant and unique green alga, given its capability of synthesizing triacylglycerol (TAG) and astaxanthin simultaneously for storage in lipid droplets (LDs). To further decipher lipid metabolism, the nitrogen deprivation (ND)-induced LDs from C. zofingiensis were isolated, purified, and subjected to proteomic analysis. Intriguingly, many C. zofingiensis LD proteins had no orthologs present in LD proteome of the model alga Chlamydomonas reinhardtii. Seven novel LD proteins (i.e., two functionally unknown proteins, two caleosins, two lipases, and one l-gulonolactone oxidase) and the major LD protein (MLDP), which were all transcriptionally up-regulated by ND, were selected for further investigation. Heterologous expression in yeast demonstrated that all tested LD proteins were localized to LDs and all except the two functionally unknown proteins enabled yeast to produce more TAG. MLDP could restore the phenotype of mldp mutant strain and enhance TAG synthesis in wild-type strain of C. reinhardtii. Although MLDP and caleosins had a comparable abundance in LDs, they responded distinctly to ND at the transcriptional level. The two lipases, instead of functioning as TAG lipases, likely recycled polar lipids to support TAG synthesis. For the first time, we reported that l-gulonolactone oxidase was abundant in LDs and facilitated TAG accumulation. Moreover, we also proposed a novel working model for C. zofingiensis LDs. Taken together, our work unravels the unique characteristics of C. zofingiensis LDs and provides insights into algal LD biogenesis and TAG synthesis, which would facilitate genetic engineering of this alga for TAG improvement.
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