ArticleBiophysical journal2019
Asymmetric Bilayers by Hemifusion: Method and Leaflet Behaviors.
Article in Biophysical journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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38 citing papers in PubMed, 60 citations in OpenAlex.
- Quantifying interleaflet coupling of phase behavior and observing anti-registered phases in asymmetric lipid bilayers.Biophysical journal · 2026Article
- Protein-free membrane fusion: A refined view of the delicate fusogenic properties of calcium.Biophysical journal · 2026Article
- Riboswitch-controlled lipid conversion enables functional membrane asymmetry in artificial cells.Communications biology · 2026Article
- Quantifying interleaflet coupling of phase behavior and observing anti-registered phases in asymmetric lipid bilayers.bioRxiv : the preprint server for biology · 2026Article
- Elucidating the mechanical properties of asymmetric membranes by direct derivation of their energetics.Faraday discussions · 2025Article
- The Importance of Bilayer Asymmetry in Biological Membranes: Insights from Model Membranes.Membranes · 2025Review
- Article
- Disruption of liquid/liquid phase separation in asymmetric GUVs prepared by hemifusion.bioRxiv : the preprint server for biology · 2024Article
- Spontaneous curvature generation by peptides in asymmetric bilayers.Journal of computational chemistry · 2024Article
- Bilayer Charge Asymmetry and Oil Residues Destabilize Membranes upon Poration.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- Protein-lipid charge interactions control the folding of outer membrane proteins into asymmetric membranes.Nature chemistry · 2023Article
- Seeing the Membrane from Both Sides Now: Lipid Asymmetry and Its Strange Consequences.Cold Spring Harbor perspectives in biology · 2023Review
- Experimentally determined leaflet-leaflet phase diagram of an asymmetric lipid bilayer.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Cholesterol, Eukaryotic Lipid Domains, and an Evolutionary Perspective of Transmembrane Signaling.Cold Spring Harbor perspectives in biology · 2023Review
- Dynamics of asymmetric membranes and interleaflet coupling as intermediates in membrane fusion.Biophysical journal · 2023Article
- Biomimetic asymmetric bacterial membranes incorporating lipopolysaccharides.Biophysical journal · 2023Article
- Quantification of Giant Unilamellar Vesicle Fusion Products by High-Throughput Image Analysis.International journal of molecular sciences · 2023Article
- Nano-scale domains in the plasma membrane are like macroscopic domains in asymmetric bilayers.Biophysical journal · 2023Review
- A Guide to Your Desired Lipid-Asymmetric Vesicles.Membranes · 2023Review
- Insights into Asymmetric Liposomes as a Potential Intervention for Drug Delivery Including Pulmonary Nanotherapeutics.Pharmaceutics · 2023Review
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2 authors at 1 institution in 1 country.
Funding
Abstract
We describe a new method to prepare asymmetric giant unilamellar vesicles (aGUVs) via hemifusion. Hemifusion of giant unilamellar vesicles and a supported lipid bilayer, triggered by calcium, promotes the lipid exchange of the fused outer leaflets mediated by lipid diffusion. We used different fluorescent dyes to monitor the inner and the outer leaflets of the unsupported aGUVs. We confirmed that almost all newly exchanged lipids in the aGUVs are found in the outer leaflet of these asymmetric vesicles. In addition, we test the stability of the aGUVs formed by hemifusion in preserving their contents during the procedure. For aGUVs prepared from the hemifusion of giant unilamellar vesicles composed of 1,2-distearoyl-sn-glycero-3-phosphocholine/1,2-dioleoyl-sn-glycero-3-phosphocholine/cholesterol = 0.39/0.39/0.22 and a supported lipid bilayer of 1,2-dioleoyl-sn-glycero-3-phosphocholine/cholesterol = 0.8/0.2, we observed the exchanged lipids to alter the bilayer properties. To access the physical and chemical properties of the asymmetric bilayer, we monitored the dye partition coefficients of individual leaflets and the generalized polarization of the fluorescence probe 6-dodecanoyl-2-[ N-methyl-N-(carboxymethyl)amino] naphthalene, a sensor for the lipid packing/order of its surroundings. For a high percentage of lipid exchange (>70%), the dye partition indicates induced-disordered and induced-ordered domains. The induced domains have distinct lipid packing/order compared to the symmetric liquid-disordered and liquid-ordered domains.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.