Evidence map›Paper›PMID 31493427›Full record

ArticleHuman pathology2019

ADAM3A copy number gains occur in a subset of conjunctival squamous cell carcinoma and its high grade precursors.

M Adelita Vizcaino, Abeer Z Tabbarah, Laura Asnaghi, Azza Maktabi, Allen O Eghrari, Divya Srikumaran, Charles G Eberhart, Fausto J Rodriguez

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Human pathology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 12 citations in OpenAlex.

  1. Review
  2. The Management of Ocular Surface Squamous Neoplasia (OSSN).International journal of molecular sciences · 2022
    Review
  3. Article
  4. Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 3 countries.

M Adelita VizcainoDepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Abeer Z TabbarahDepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Pathology, American University of Beirut Medical Center, Beirut, Lebanon.
Laura AsnaghiDepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Azza MaktabiKing Khaled Eye Specialist Hospital, Riyadh, Saudi Arabia.
Allen O EghrariWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Divya SrikumaranWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Charles G EberhartDepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Sydney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Fausto J RodriguezDepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Sydney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: frodrig4@jhmi.edu.
Johns Hopkins Medicine · USJohns Hopkins University · USAmerican University of Beirut Medical Center · LBKing Khaled Eye Specialist Hospital · SA

Funding

Translational Research Central ServicesP30CA006973 · NCI · JOHNS HOPKINS UNIVERSITY · PI ALAN KEITH MEEKER · 1985 to 2026
$208.6M
NCI NIH HHS P30 CA006973
6 · The paper itself

Abstract

Conjunctival squamous cell carcinoma (cSCC) and its precursors are among the most frequent ocular surface neoplasms worldwide. Copy gain of 8p11.22 and ADAM3A overexpression have been recently identified in invasive cSCC. We sought to study copy number gains using fluorescent in situ hybridization (FISH) in cSCC and the spectrum of precursor lesions. A total of 54 cases conjunctival squamous intraepithelial neoplasia (CIN), carcinoma in situ (CIS), or cSCC were studied using FISH with an ADAM3A (8p11 locus) probe and a chromosome 8 (Chr 8) centromere reference probe. Eighty one percent (44/54) of the cases presented in men and 19% (10/54) in women. The age at presentation ranged from 12 to 94 years (mean 65.5 years). Severe CIN was diagnosed in 45% (24/54) of the cases, followed by CIS in 31% (17/54), moderate CIN in 15% (8/54), invasive cSCC in 7% (4/54), and mild CIN in 2% (1/54). Nine (of 54) (17%) cases harbored ADAM3A or Chr 8 gains, with one of these cases demonstrating high level amplification. All ADAM3A alterations were restricted to high-grade lesions, including 2/17 (12%) cCIS, 1/4 (24%) cSCC, 5/24 (20%) severe CIN and 1/8 (12%) moderate CIN. Monosomy 8 was detected in 2 (4%) cases. No ADAM3A alterations were detected in non-neoplastic controls. Gains of ADAM3A/chromosome 8 occur in a subset of cSCC and its precursors. Alterations were present in high-grade lesions, sparing non-neoplastic conjunctiva and absent in tested controls. Thus, the specificity of this alteration as a biomarker for ocular SCC deserves further study.

Indexed as

Gene AmplificationGene DosageADAM ProteinsAdolescentAdultAgedAged, 80 and overBiomarkers, TumorChildConjunctival NeoplasmsFemaleHumansIn Situ Hybridization, FluorescenceMaleMiddle AgedNeoplasm GradingADAM3A protein, humanADAM ProteinsBiomarkers, TumorADAM3AConjunctivaConjunctival squamous intraepithelial neoplasiaOcular surface carcinomaSquamous cell carcinoma

Identifiers

PMID31493427
PMCPMC6917831
OpenAlexW2971795818

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.