ArticleJournal of molecular biology2020
The Solution Structures and Interaction of SinR and SinI: Elucidating the Mechanism of Action of the Master Regulator Switch for Biofilm Formation in Bacillus subtilis.
Article in Journal of molecular biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- MeeY and YybP, two proteins regulated by manganese-sensing riboswitches, are required for Bacillus subtilis biofilm formation.NPJ biofilms and microbiomes · 2026Article
- The major toxins of Bacillus cereus - Current knowledge on distribution, regulation and mechanism of action.EXCLI journal · 2026Review
- Movement Mechanisms Harness Lévy Flight for Energy-Efficient Wastewater Treatment in Microalgae-Bacteria Systems.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- The SinR·SlrR Heteromer Attenuates Transcription of a Long Operon of Flagellar Genes in Bacillus subtilis.Journal of molecular biology · 2025Article
- A novel regulation on the developmental checkpoint protein Sda that controls sporulation and biofilm formation inJournal of bacteriology · 2025Article
- Adaptive laboratory evolution reveals regulators involved in repressing biofilm development as key players inmSystems · 2024Article
- XRE transcription factors conserved in Caulobacter and φCbK modulate adhesin development and phage production.PLoS genetics · 2023Article
- Efficient Inhibition of Bacterial Biofilm Through Interference of Protein-Protein Interaction of Master Regulator Proteins: a Proof of Concept Study with SinR- SinI Complex of Bacillus subtilis.Applied biochemistry and biotechnology · 2023Article
- The coordinated population redistribution betweenBiofilm · 2022Article
- Structural basis of flagellar motility regulation by the MogR repressor and the GmaR antirepressor in Listeria monocytogenes.Nucleic acids research · 2022Article
- Transcriptomic Analysis Reveals the Role of tmRNA on Biofilm Formation inMicroorganisms · 2022Article
- Crystal structure report of the ImmR transcriptional regulator DNA-binding domain of the Bacillus subtilis ICEBs1 transposon.Scientific reports · 2022Article
- Loss of an Intimin-Like Protein Encoded on a Uropathogenic E. coli Pathogenicity Island Reduces Inflammation and Affects Interactions with the Urothelium.Infection and immunity · 2022Article
- Structural and functional characterization of the bacterial biofilm activator RemA.Nature communications · 2021Article
- Bacillus subtilis biofilm formation and social interactions.Nature reviews. Microbiology · 2021Review
- Site-directed mutagenesis of the quorum-sensing transcriptional regulator SinR affects the biosynthesis of menaquinone in Bacillus subtilis.Microbial cell factories · 2021Article
- The Food Poisoning Toxins ofToxins · 2021Review
- Spo0A SuppressesmSphere · 2020Article
- The YmdB protein regulates biofilm formation dependent on the repressor SinR in Bacillus cereus 0-9.World journal of microbiology & biotechnology · 2020Article
- The Large pBS32/pLS32 Plasmid of Ancestral Bacillus subtilis.Journal of bacteriology · 2020Review
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Authors and funding
11 authors.
Funding
Abstract
Bacteria have developed numerous protection strategies to ensure survival in harsh environments, with perhaps the most robust method being the formation of a protective biofilm. In biofilms, bacterial cells are embedded within a matrix that is composed of a complex mixture of polysaccharides, proteins, and DNA. The gram-positive bacterium Bacillus subtilis has become a model organism for studying regulatory networks directing biofilm formation. The phenotypic transition from a planktonic to biofilm state is regulated by the activity of the transcriptional repressor, SinR, and its inactivation by its primary antagonist, SinI. In this work, we present the first full-length structural model of tetrameric SinR using a hybrid approach combining high-resolution solution nuclear magnetic resonance (NMR), chemical cross-linking, mass spectrometry, and molecular docking. We also present the solution NMR structure of the antagonist SinI dimer and probe the mechanism behind the SinR-SinI interaction using a combination of biochemical and biophysical techniques. As a result of these findings, we propose that SinI utilizes a residue replacement mechanism to block SinR multimerization, resulting in diminished DNA binding and concomitant decreased repressor activity. Finally, we provide an evidence-based mechanism that confirms how disruption of the SinR tetramer by SinI regulates gene expression.
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