Evidence map›Paper›PMID 31493190›Full record

ArticleMolecular and cellular biochemistry2019

Cytotoxic and antiproliferative effects of thymoquinone on rat C6 glioma cells depend on oxidative stress.

N G Krylova, M S Drobysh, G N Semenkova, T A Kulahava, S V Pinchuk, O I Shadyro

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular and cellular biochemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
4.1field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 22 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. An Insight into Emerging Phytocompounds for Glioblastoma Multiforme Therapy.Cardiovascular & hematological agents in medicinal chemistry · 2024
    Review
  5. Review
  6. Review
  7. Article
  8. Review
  9. IP-Se-06, a Selenylated Imidazo[1,2-Oxidative medicine and cellular longevity · 2022
    Article
  10. Review
  11. Review
  12. Review
  13. Review
  14. Article
  15. Review
  16. Frontiers in neuroscience · 2020
    Article
  17. Recent Progress on Chemical Constituents and Pharmacological Effects of the GenusEvidence-based complementary and alternative medicine : eCAM · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

N G KrylovaDepartment of Biophysics, Faculty of Physics, Belarusian State University, 4 Nezavisimosti ave., 220030, Minsk, Belarus.
M S DrobyshDepartment of Radiation Chemistry and Pharmaceutical Technologies, Faculty of Chemistry, Belarusian State University, 14 Leningradskaya st., 220030, Minsk, Belarus.
G N SemenkovaDepartment of Radiation Chemistry and Pharmaceutical Technologies, Faculty of Chemistry, Belarusian State University, 14 Leningradskaya st., 220030, Minsk, Belarus.
T A KulahavaDepartment of Biophysics, Faculty of Physics, Belarusian State University, 4 Nezavisimosti ave., 220030, Minsk, Belarus. tatyana_kulagova@tut.by.
S V PinchukInstitute of Biophysics and Cell Engineering of National Academy of Sciences of Belarus, 27 Academicheskaya st., 220072, Minsk, Belarus.
O I ShadyroDepartment of Radiation Chemistry and Pharmaceutical Technologies, Faculty of Chemistry, Belarusian State University, 14 Leningradskaya st., 220030, Minsk, Belarus.
Belarusian State University · BYInstitute of Biophysics and Cell Engineering · BY

Funding

Belarusian Republican Foundation for Fundamental Research M17M-092Ministry of Education of Belarus 20161385
6 · The paper itself

Abstract

Thymoquinone (TQ) is a highly perspective chemotherapeutic agent against gliomas and glioblastomas because of its ability to cross the blood-brain barrier and its selective cytotoxicity for glioblastoma cells compared to primary astrocytes. Here, we tested the hypothesis that TQ-induced mild oxidative stress provokes C6 glioma cell apoptosis through redox-dependent alteration of MAPK proteins. We showed that low concentrations of TQ (20-50 μM) promoted cell-cycle arrest and induced hydrogen peroxide generation as a result of NADH-quinone oxidoreductase 1-catalyzed two-electron reduction of this quinone. Similarly, low concentrations of TQ efficiently conjugated intracellular GSH disturbing redox state of glioma cells and provoking mitochondrial dysfunction. We demonstrated that high concentrations of TQ (70-100 μM) induced reactive oxygen species generation due to its one-electron reduction. TQ provoked apoptosis in C6 glioma cells through mitochondrial potential dissipation and permeability transition pore opening. The identified TQ modes of action on C6 glioma cells open up the possibility of considering it as a promising agent to enhance the sensitivity of cancer cells to standard chemotherapeutic drugs.

Indexed as

AnimalsApoptosisBenzoquinonesCell Line, TumorCell ProliferationCell SurvivalGliomaMAP Kinase Signaling SystemMitochondriaOxidation-ReductionOxidative StressPhosphatidylinositol 3-KinasesRatsBenzoquinonesPhosphatidylinositol 3-KinasesthymoquinoneApoptosisGliomaMitochondrial dysfunctionReactive oxygen speciesThymoquinone

Identifiers

PMID31493190
OpenAlexW2972077946

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.