Evidence map›Paper›PMID 31492906›Full record

ArticleScientific reports2019

Extracellular vesicle-coupled miRNA profiles in follicular fluid of cows with divergent post-calving metabolic status.

Tsige Hailay, Michael Hoelker, Mikhael Poirier, Samuel Gebremedhn, Franca Rings, Mohammed Saeed-Zidane, Dessie Salilew-Wondim, Christina Dauben, Ernst Tholen, Christiane Neuhoff and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed
2.4field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 55 citations in OpenAlex.

  1. Article
  2. Review
  3. The Influence of Ovarian-Derived Extracellular Vesicles in Reproduction.Advances in anatomy, embryology, and cell biology · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 1 institution in 1 country.

Tsige HailayInstitute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Bonn, Germany.
Michael HoelkerInstitute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Bonn, Germany.
Mikhael PoirierInstitute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Bonn, Germany.
Samuel GebremedhnInstitute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Bonn, Germany.ORCID http://orcid.org/0000-0002-9977-2347
Franca RingsInstitute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Bonn, Germany.
Mohammed Saeed-ZidaneInstitute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Bonn, Germany.
Dessie Salilew-WondimInstitute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Bonn, Germany.
Christina DaubenInstitute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Bonn, Germany.
Ernst TholenInstitute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Bonn, Germany.
Christiane NeuhoffInstitute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Bonn, Germany.
Karl SchellanderInstitute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Bonn, Germany.
Dawit TesfayeInstitute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Bonn, Germany. tesfaye@itw.uni-bonn.de.
University of Bonn · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Most high-yielding dairy cows enter a state of negative energy balance (NEB) during early lactation. This, in turn, results in changes in the level of various metabolites in the blood and follicular fluid microenvironment which contributes to disturbed fertility. Extracellular vesicles (EVs) are evolutionarily conserved communicasomes that transport cargo of miRNA, proteins and lipids. EV-coupled miRNAs have been reported in follicular fluid. However, the association between postpartum NEB and EV-coupled miRNA signatures in follicular fluid is not yet known. Energy balance analysis in lactating cows shortly after post-calving revealed that the majority of the cows exhibited transiently negative energy balance levels, whereas the remaining cows exhibited either consistently negative or consistently positive energy levels. Metabolic status was associated with EV-coupled miRNA composition in the follicular fluid. Cows experiencing NEB showed reduced expression of a large number of miRNAs while cows with positive energy balances primarily exhibited elevated expression of EV-coupled miRNAs. The miRNAs that were suppressed under NEB were found to be involved in various metabolic pathways. This is the first study to reveal the presence of an association between EV-coupled miRNA in follicular fluid and metabolic stress in dairy cows. The involvement of differentially expressed miRNAs in various pathways associated with follicular growth and oocyte maturation suggest the potential involvement of specific follicular miRNAs in oocyte developmental competence, which may partially explain reduced fertility in cows due to post-calving metabolic stress.

Indexed as

Gene Expression ProfilingAnimalsCattleEnergy MetabolismExtracellular VesiclesFemaleFollicular FluidLactationMetabolomeMicroRNAsPostpartum PeriodMicroRNAs

Identifiers

PMID31492906
PMCPMC6731312
OpenAlexW2972096429

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.