ArticleThe Journal of experimental medicine2019
B cell dissemination patterns during the germinal center reaction revealed by whole-organ imaging.
Article in The Journal of experimental medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Germinal Center Selection and Plasma Cell Differentiation.Immunological reviews · 2026Review
- MINGL Quantifies Borders, Gradients, and Heterogeneity in Multicellular Tissue Organization.bioRxiv : the preprint server for biology · 2026Article
- The normal human lymph node cell classification and landscape defined by high-dimensional spatial proteomics.PloS one · 2026Article
- Of Tingible Bodies and Starry Skies: Control of Normal and Malignant Tissues Through Apoptotic Cell-Dependent Communication (ACDC).Advances in experimental medicine and biology · 2025Review
- SMARCA5-mediated chromatin remodeling is required for germinal center formation.The Journal of experimental medicine · 2024Article
- Article
- Spatial immunophenotyping using multiplexed imaging of immune follicles in secondary lymphoid tissues.PNAS nexus · 2024Article
- Harnessing the potential of the NALT and BALT as targets for immunomodulation using engineering strategies to enhance mucosal uptake.Frontiers in immunology · 2024Review
- Recycling of memory B cells between germinal center and lymph node subcapsular sinus supports affinity maturation to antigenic drift.Nature communications · 2022Article
- Intravital three-photon microscopy allows visualization over the entire depth of mouse lymph nodes.Nature immunology · 2022Article
- Complete Visualization of T Follicular Helper Cells in Germinal Centers by Light Sheet Fluorescence Microscopy.Methods in molecular biology (Clifton, N.J.) · 2022Article
- LigA formulated in AS04 or Montanide ISA720VG induced superior immune response compared to alum, which correlated to protective efficacy in a hamster model of leptospirosis.Frontiers in immunology · 2022Article
- Article
- The path of the T-bet-ian CD8Nature immunology · 2021Article
- Acute Lymph Node Slices Are a Functional Model System to Study Immunity Ex Vivo.ACS pharmacology & translational science · 2021Article
- Characterization of Immunological Niches within Peyer's Patches byBio-protocol · 2020Article
- Potential Application of T-Follicular Regulatory Cell Therapy in Transplantation.Frontiers in immunology · 2020Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Germinal centers (GCs) are sites wherein B cells proliferate and mutate their immunoglobulins in the dark zone (DZ), followed by affinity-based selection in the light zone (LZ). Here, we mapped the location of single B cells in the context of intact lymph nodes (LNs) throughout the GC response, and examined the role of BCR affinity in dictating their position. Imaging of entire GC structures and proximal single cells by light-sheet fluorescence microscopy revealed that individual B cells that previously expressed AID are located within the LN cortex, in an area close to the GC LZ. Using in situ photoactivation, we demonstrated that B cells migrate from the LZ toward the GC outskirts, while DZ B cells are confined to the GC. B cells expressing very-low-affinity BCRs formed GCs but were unable to efficiently disperse within the follicles. Our findings reveal that BCR affinity regulates B cell positioning during the GC response.
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Registered trials
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