ArticleJournal of the American Society of Nephrology : JASN2019
A Toolbox to Characterize Human Induced Pluripotent Stem Cell-Derived Kidney Cell Types and Organoids.
Article in Journal of the American Society of Nephrology : JASN, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 66 citations in OpenAlex.
- Application of spatial transcriptomics across organoids for a high-resolution, spatial whole-transcriptome benchmarking dataset.iScience · 2026Article
- Classification of indeterminate and off-target cell types within human kidney organoid differentiation.iScience · 2026Article
- In vitro plasticity between ureteric epithelial and distal nephron identity and maturity is controlled by extracellular signals.Developmental biology · 2026Article
- Developmentally inspired synthetic kidney engineering.Nature biotechnology · 2026Review
- Synthetic budding morphogenesis by optogenetic receptor tyrosine kinase signaling.bioRxiv : the preprint server for biology · 2026Article
- Human stem cell-based embryo models: from self-organization to recapitulating development.Frontiers in cell and developmental biology · 2026Review
- Mitochondrial organization in the developing proximal tubule is controlled by LRRK2.Nature communications · 2025Article
- Controlling nephron precursor differentiation to generate proximal-biased kidney organoids with emerging maturity.Nature communications · 2025Article
- Axial nephron fate switching demonstrates a plastic system tunable on demand.Nature communications · 2025Article
- Single cell transcriptomics of human kidney organoid endothelium reveals vessel growth processes and arterial maturation upon transplantation.NPJ Regenerative medicine · 2025Article
- Highly parallel production of designer organoids by mosaic patterning of progenitors.Cell systems · 2024Article
- Kidney organoids reveal redundancy in viral entry pathways during ACE2-dependent SARS-CoV-2 infection.Journal of virology · 2024Article
- Nephron progenitors rhythmically alternate between renewal and differentiation phases that synchronize with kidney branching morphogenesis.bioRxiv : the preprint server for biology · 2024Article
- Navigating the kidney organoid: insights into assessment and enhancement of nephron function.American journal of physiology. Renal physiology · 2023Review
- Rho/ROCK activity tunes cell compartment segregation and differentiation in nephron-forming niches.bioRxiv : the preprint server for biology · 2023Article
- Generation of proximal tubule-enhanced kidney organoids from human pluripotent stem cells.Nature protocols · 2023Review
- Genetically engineering endothelial niche in human kidney organoids enables multilineage maturation, vascularization and de novo cell types.bioRxiv : the preprint server for biology · 2023Article
- Lymphoid cell development from fetal hematopoietic progenitors and human pluripotent stem cells.Immunological reviews · 2023Review
- 3D Bioprinting for Next-Generation Personalized Medicine.International journal of molecular sciences · 2023Review
- Review
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 3 countries.
Funding
Abstract
backgroundThe generation of reporter lines for cell identity, lineage, and physiologic state has provided a powerful tool in advancing the dissection of mouse kidney morphogenesis at a molecular level. Although use of this approach is not an option for studying human development
methodsWe used CRISPR/Cas9 gene editing to generate ten fluorescence reporter iPSC lines designed to identify nephron progenitors, podocytes, proximal and distal nephron, and ureteric epithelium. Directed differentiation to kidney organoids was performed according to published protocols. Using immunofluorescence and live confocal microscopy, flow cytometry, and cell sorting techniques, we investigated organoid patterning and reporter expression characteristics.
resultsEach iPSC reporter line formed well patterned kidney organoids. All reporter lines showed congruence of endogenous gene and protein expression, enabling isolation and characterization of kidney cell types of interest. We also demonstrated successful application of reporter lines for time-lapse imaging and mouse transplantation experiments.
conclusionsWe generated, validated, and applied a suite of fluorescence iPSC reporter lines for the study of morphogenesis within human kidney organoids. This fluorescent iPSC reporter toolbox enables the visualization and isolation of key populations in forming kidney organoids, facilitating a range of applications, including cellular isolation, time-lapse imaging, protocol optimization, and lineage-tracing approaches. These tools offer promise for enhancing our understanding of this model system and its correspondence with human kidney morphogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.