Evidence map›Paper›PMID 31492192›Full record

ArticleBMC veterinary research2019

Novel polymerase spiral reaction assay for the visible molecular detection of porcine circovirus type 3.

Jun Ji, Xin Xu, Xueyu Wang, Kejing Zuo, Zhili Li, Chaoliang Leng, Yunchao Kan, Lunguang Yao, Yingzuo Bi

Open access · goldAbstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
2.3field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 24 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Review
  7. Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Jun JiHenan Provincial Engineering Laboratory of Insect Bio-reactor and Henan Key Laboratory of Ecological Security for Water Source Region of Mid-line of South-to-North, Nanyang Normal University, 1638 Wolong Road, Nanyang, Hena, 473061, People's Republic of China. jijun020@126.com.ORCID http://orcid.org/0000-0001-9511-4163
Xin XuHenan Provincial Engineering Laboratory of Insect Bio-reactor and Henan Key Laboratory of Ecological Security for Water Source Region of Mid-line of South-to-North, Nanyang Normal University, 1638 Wolong Road, Nanyang, Hena, 473061, People's Republic of China.
Xueyu WangHenan Provincial Engineering Laboratory of Insect Bio-reactor and Henan Key Laboratory of Ecological Security for Water Source Region of Mid-line of South-to-North, Nanyang Normal University, 1638 Wolong Road, Nanyang, Hena, 473061, People's Republic of China.
Kejing ZuoVeterinary Laboratory, Guangzhou Zoo, Guangzhou, 510642, People's Republic of China.
Zhili LiCollege of Animal Science, South China Agricultural University, Guangzhou, 510642, People's Republic of China.
Chaoliang LengHenan Provincial Engineering Laboratory of Insect Bio-reactor and Henan Key Laboratory of Ecological Security for Water Source Region of Mid-line of South-to-North, Nanyang Normal University, 1638 Wolong Road, Nanyang, Hena, 473061, People's Republic of China.
Yunchao KanHenan Provincial Engineering Laboratory of Insect Bio-reactor and Henan Key Laboratory of Ecological Security for Water Source Region of Mid-line of South-to-North, Nanyang Normal University, 1638 Wolong Road, Nanyang, Hena, 473061, People's Republic of China.
Lunguang YaoHenan Provincial Engineering Laboratory of Insect Bio-reactor and Henan Key Laboratory of Ecological Security for Water Source Region of Mid-line of South-to-North, Nanyang Normal University, 1638 Wolong Road, Nanyang, Hena, 473061, People's Republic of China. lunguangyao@163.com.
Yingzuo BiCollege of Animal Science, South China Agricultural University, Guangzhou, 510642, People's Republic of China.
Nanyang Normal University · CNSouth China Agricultural University · CNGuangzhou Zoo · CN

Funding

National Natural Science Foundation of China 31870917
6 · The paper itself

Abstract

backgroundPorcine circovirus type 3 (PCV3) is a newly emerging circovirus that might be associated with porcine dermatitis and nephropathy syndrome, reproductive failure, and cardiac and multisystemic inflammation. To aid the prevention and control of the infectious disease caused by PCV3, we developed a novel isothermal amplification assay using polymerase spiral reaction (PSR), which allows the visual detection of preserved strains and clinical samples.

resultsThis assay precisely amplified the PCV3 genome with the use of a water bath at 62 °C for 50 min. The detection limit was found to be 1.13 × 10

conclusionsThese results indicated that the PSR assay provides an accurate and rapid method for the detection of PCV3 with high sensitivity and specificity. It is particularly suited for use in a simple laboratory setting without a thermal cycler or gel electrophoresis equipment.

Indexed as

AnimalsCircovirusDNA-Directed DNA PolymeraseNucleic Acid Amplification TechniquesSensitivity and SpecificitySwineSwine DiseasesDNA-Directed DNA PolymeraseMolecular detectionPCV3Polymerase spiral reactionVisible detection

Identifiers

PMID31492192
PMCPMC6731610
OpenAlexW2971358552

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.