Evidence map›Paper›PMID 31489562›Full record

ArticleJournal of the American Society for Mass Spectrometry2019

Electrospray Photochemical Oxidation of Proteins.

Remilekun O Lawal, Fabrizio Donnarumma, Kermit K Murray

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of the American Society for Mass Spectrometry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Remilekun O LawalDepartment of Chemistry, Louisiana State University, Baton Rouge, LA, 70803, USA.
Fabrizio DonnarummaDepartment of Chemistry, Louisiana State University, Baton Rouge, LA, 70803, USA.
Kermit K MurrayDepartment of Chemistry, Louisiana State University, Baton Rouge, LA, 70803, USA. kkmurray@lsu.edu.ORCID http://orcid.org/0000-0002-4976-2263

Funding

Two Laser Ablation Electrospray Ionization Mass Spectrometry ImagingR21EB023110 · NIBIB · LOUISIANA STATE UNIV A&M COL BATON ROUGE · PI MURRAY, KERMIT KING · 2017 to 2018
$393k
NIBIB NIH HHS R21 EB023110NIH HHS R21 EB-023110
6 · The paper itself

Abstract

Photooxidation of peptides and proteins by pulsed ultraviolet laser irradiation of an electrospray in the ion source of a mass spectrometer was demonstrated. A 193-nm excimer laser at 1.5-mJ pulse energy was focused with a cylindrical lens at the exit of a nanoelectrospray capillary and ions were sampled into a quadrupole time-of-flight mass spectrometer. A solution containing a peptide or protein and hydrogen peroxide was infused into the spray at a flow rate of 1 μL/min using a syringe pump. The laser creates OH radicals directly in the spray which modify biomolecules within the spray droplet. These results indicate that photochemical oxidation of proteins can be initiated directly within electrospray droplets and detected by mass spectrometry.

Indexed as

Hydrogen PeroxideNanotechnologyOxidation-ReductionPeptidesPhotochemical ProcessesProteinsSpectrometry, Mass, Electrospray IonizationUltraviolet RaysHydrogen PeroxidePeptidesProteinsElectrosprayExcimer OnlineFPOPPhotochemical oxidationUltraviolet

Identifiers

PMID31489562
PMCPMC6832858

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.