ArticleScientific reports2019
Freezability biomarkers in bull epididymal spermatozoa.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 51 citations in OpenAlex.
- Recent advances in cryotolerance biomarkers for semen preservation in frozen form-A systematic review.PloS one · 2024Pooled it
- Epididymosome-Supplemented Extender Induces Changes in Morpho-Functional Traits and microRNA Levels of Post-Thaw Sperm and Improves Embryo Developmental Potential.Molecular reproduction and development · 2026Article
- Cryotolerance of brown bear (Ursus arctos) sperm depends on sperm origin: insights into sperm quality and proteomic profiles.Scientific reports · 2026Article
- Optimizing South African giraffe Epididymal sperm preservation techniques".Veterinary and animal science · 2026Article
- Integrated Proteomics and Metabolomics Reveal Spermine Enhances Sperm Freezability via Antioxidant Pathways.Antioxidants (Basel, Switzerland) · 2025Article
- Improving Ram Semen Low Cryotolerance by Replacing the Seminal Plasma With That of High-Cryotolerant Rams or Extender.Veterinary medicine and science · 2025Article
- Overcoming ice: cutting-edge materials and advanced strategies for effective cryopreservation of biosample.Journal of nanobiotechnology · 2025Review
- Extender development for optimal cryopreservation of buck sperm to increase reproductive efficiency of goats.Frontiers in veterinary science · 2025Review
- Biomarker-based human and animal sperm phenotyping: the good, the bad and the ugly†.Biology of reproduction · 2024Review
- iTRAQ-based comparative proteomics reveal an enhancing role of PRDX6 in the freezability of Mediterranean buffalo sperm.BMC genomics · 2023Article
- Molecular Markers: A New Paradigm in the Prediction of Sperm Freezability.International journal of molecular sciences · 2023Review
- Cryopreservation of Semen in Domestic Animals: A Review of Current Challenges, Applications, and Prospective Strategies.Animals : an open access journal from MDPI · 2022Review
- Expression ofInternational journal of molecular sciences · 2022Article
- Evaluation of lipidomic change in goat sperm after cryopreservation.Frontiers in veterinary science · 2022Article
- An Exploration of Current and Perspective Semen Analysis and Sperm Selection for Livestock Artificial Insemination.Animals : an open access journal from MDPI · 2021Review
- Macro- and microelements in serum and seminal plasma as biomarkers for bull sperm cryotolerance.Acta veterinaria Scandinavica · 2021Article
- Bioinformatic Approach to Unveil Key Differentially Expressed Proteins in Human Sperm After Slow and Rapid Cryopreservation.Frontiers in cell and developmental biology · 2021Article
- Protein Identification of Spermatozoa and Seminal Plasma in Bottlenose Dolphin (Frontiers in cell and developmental biology · 2021Article
- ProAKAP4 as Novel Molecular Marker of Sperm Quality in Ram: An Integrative Study in Fresh, Cooled and Cryopreserved Sperm.Biomolecules · 2020Article
- Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality.International journal of molecular sciences · 2020Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sperm cryopreservation is an important tool for storing genetic traits and assisted reproduction techniques. Several studies have developed semen cryopreservation protocols. However, the sperm proteome is different between ejaculated and epididymal spermatozoa and little is known about cryopreservation effects on epididymal spermatozoa. Therefore, our study aimed to (i) investigate the differences of sperm parameters based on the freezing tolerance of spermatozoa and (ii) identify potential markers to predict the freezability of bull epididymal spermatozoa. Our preliminary study demonstrated that spermatozoa from individual bulls differ in cryopreservation freezability. We categorized spermatozoa into high freezing-tolerant spermatozoa and low freezing-tolerant spermatozoa group based on sperm motility after freezing/thawing. We evaluated several sperm functional parameters, including sperm motility/motion kinematics, sperm speed parameters, viability, mitochondrial activity, and capacitation status. Our results demonstrated that motility, sperm speed parameters, viability, and mitochondrial membrane potential had significant differences between the two groups but motion kinematics and capacitation status did not. In addition, the concentration of three proteins - glutathione s-transferase mu 5, voltage-dependent anion-selective channel protein 2, and ATP synthase subunit beta, differed between both groups. Thus, our research highlighted differences in bull epididymal spermatozoa freezability upon cryopreservation and these proteins might be useful markers to select high freezing-tolerant epididymal spermatozoa.
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