Evidence map›Paper›PMID 31488703›Full record

ArticleScience (New York, N.Y.)2019

CRISPR-mediated live imaging of genome editing and transcription.

Haifeng Wang, Muneaki Nakamura, Timothy R Abbott, Dehua Zhao, Kaiwen Luo, Cordelia Yu, Cindy M Nguyen, Albert Lo, Timothy P Daley, Marie La Russa and 2 more

Open access · greenAbstract readVideo-Audio Media
PubMed Full text at PMC Publisher
In one paragraph

Article in Science (New York, N.Y.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 157 papers.

0numbers the graph read from it
0cells of the map it votes in
157citing papers in PubMed
12.6field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

157 citing papers in PubMed, 298 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Review
  6. Article
  7. Article
  8. Review
  9. Review
  10. Article
  11. Review
  12. Review
  13. Review
  14. Review
  15. Review
  16. Article
  17. Article
  18. Review
  19. Review
  20. Review

97 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 2 countries.

Haifeng WangDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.ORCID 0000-0003-4792-2005
Muneaki NakamuraDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Timothy R AbbottDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.ORCID 0000-0001-7404-811X
Dehua ZhaoDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Kaiwen LuoDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.ORCID 0000-0002-1597-1219
Cordelia YuDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Cindy M NguyenDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.ORCID 0000-0001-6281-2125
Albert LoDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.ORCID 0000-0003-4838-9433
Timothy P DaleyDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.ORCID 0000-0002-6667-1654
Marie La RussaDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Yanxia LiuDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.ORCID 0000-0002-6550-3624
Lei S QiDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA. stanley.qi@stanford.edu.ORCID 0000-0002-3965-3223
Stanford University · USZhejiang University-University of Edinburgh Institute · CN

Funding

Hi-resolution dynamic imaging of chromosomes in single cells by combined CRISPR imaging and sequential FISHU01EB021240 · NIBIB · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI CAI, LONG, QI, LEI STANLEY · 2015 to 2017
$1.4M
NIBIB NIH HHS U01 EB021240
6 · The paper itself

Abstract

We report a robust, versatile approach called CRISPR live-cell fluorescent in situ hybridization (LiveFISH) using fluorescent oligonucleotides for genome tracking in a broad range of cell types, including primary cells. An intrinsic stability switch of CRISPR guide RNAs enables LiveFISH to accurately detect chromosomal disorders such as Patau syndrome in prenatal amniotic fluid cells and track multiple loci in human T lymphocytes. In addition, LiveFISH tracks the real-time movement of DNA double-strand breaks induced by CRISPR-Cas9-mediated editing and consequent chromosome translocations. Finally, by combining Cas9 and Cas13 systems, LiveFISH allows for simultaneous visualization of genomic DNA and RNA transcripts in living cells. The LiveFISH approach enables real-time live imaging of DNA and RNA during genome editing, transcription, and rearrangements in single cells.

Indexed as

CRISPR-Cas SystemsGene EditingCell Line, TumorDNADNA Breaks, Double-StrandedGenetic VectorsHEK293 CellsHumansIn Situ Hybridization, FluorescenceMicroscopy, FluorescenceMolecular ImagingRNARNA, Guide, CRISPR-Cas SystemsT-LymphocytesDNARNARNA, Guide, CRISPR-Cas Systems

Identifiers

PMID31488703
PMCPMC13030913
OpenAlexW2971493710

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.