Evidence map›Paper›PMID 31488061›Full record

ArticleBMC microbiology2019

AcrAB-TolC efflux pump system plays a role in carbapenem non-susceptibility in Escherichia coli.

Shiela Chetri, Deepshikha Bhowmik, Deepjyoti Paul, Piyush Pandey, Debadatta Dhar Chanda, Atanu Chakravarty, Debajyoti Bora, Amitabha Bhattacharjee

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In one paragraph

Article in BMC microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
55citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

55 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. UropathogenicAntibiotics (Basel, Switzerland) · 2026
    Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Molecular Insights into Carbapenem Resistance inInternational journal of molecular sciences · 2026
    Review
  8. Article
  9. Molecular epidemiology and mechanisms ofFrontiers in microbiology · 2026
    Article
  10. Article
  11. Article
  12. Review
  13. Review
  14. Article
  15. Review
  16. Article
  17. Review
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Shiela ChetriDepartment of Microbiology, Assam University, Silchar, Assam, India.
Deepshikha BhowmikDepartment of Microbiology, Assam University, Silchar, Assam, India.
Deepjyoti PaulDepartment of Microbiology, Assam University, Silchar, Assam, India.
Piyush PandeyDepartment of Microbiology, Assam University, Silchar, Assam, India.
Debadatta Dhar ChandaDepartment of Microbiology, Silchar Medical College and Hospital, Silchar, India.
Atanu ChakravartyDepartment of Microbiology, Silchar Medical College and Hospital, Silchar, India.
Debajyoti BoraDepartment of Statistics, Dibrugarh University, Dibrugarh, India.
Amitabha BhattacharjeeDepartment of Microbiology, Assam University, Silchar, Assam, India. ab0404@gmail.com.ORCID 0000-0002-3111-9835

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundEfflux pump mediated antibiotic resistance is an unnoticed and undetected mechanism in clinical microbiology laboratory. RND efflux systems are known for aminoglycoside and tetracycline resistance whereas their role in carbapenem non-susceptibility is not established. The study was undertaken to investigate the role of efflux pump in providing resistance against carbapenems and their response against concentration gradient carbapenem stress on the transcriptional level of the AcrAB gene in the clinical isolates of Escherichia coli from a tertiary referral hospital of Northeast India.

resultsOut of 298 non-susceptible Escherichia coli isolates 98 isolates were found to have efflux pump mediated carbapenem non-susceptibility. Among them thirty-five were non carbapenemase producers and their expressional levels were verified using qRT-PCR under concentration gradient carbapenem stress. In this study, a strong correlation between ertapenem resistance and AcrA overexpression was observed which has not been reported previously. Further, it was observed that imipenem stress increased AcrB expression in Escherichia coli which holds the novelty of this study. Additionally, the transcription of AcrR was insistently increased which is much higher than the transcriptional level of AcrA under concentration gradient carbapenem stress condition.

conclusionThe study established that AcrAB pump is a relevant antibiotic resistance determinant in bacterial pathogen, has an important role in developing resistance against carbapenem group of antibiotics.

Indexed as

Drug Resistance, BacterialAnti-Bacterial AgentsBacterial Proteinsbeta-LactamasesCarbapenemsCarrier ProteinsEscherichia coliEscherichia coli InfectionsEscherichia coli ProteinsHumansIndiaMicrobial Sensitivity TestsTertiary Care CentersTranscription, GeneticAcrAB-TolC protein, E coliAnti-Bacterial AgentsBacterial Proteinsbeta-LactamasescarbapenemaseCarbapenemsCarrier ProteinsEscherichia coli ProteinsAcrAB-TolCCarbapenemsCCCPEscherichia coliReal-time PCR

Identifiers

PMID31488061
PMCPMC6727511

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.