ArticleBMC microbiology2019
Synergistic action of SPI-1 gene expression in Salmonella enterica serovar typhimurium through transcriptional crosstalk with the flagellar system.
Article in BMC microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed.
- HilE mediates motility thermoregulation in typhoidal Salmonella serovars at elevated physiological temperatures.PLoS pathogens · 2025Article
- SanA is an inner membrane protein mediatingMicrobiology spectrum · 2025Article
- N-Acetyl cysteine exhibits antimicrobial and anti-virulence activity against Salmonella enterica.PloS one · 2025Article
- Unravelling the Roles of Bacterial Nanomachines Bistability in Pathogens' Life Cycle.Microorganisms · 2024Review
- Transcriptome Analysis Reveals Cross-Talk between the Flagellar Transcriptional Hierarchy and Secretion System inInternational journal of molecular sciences · 2024Article
- Persistent Salmonella infections in humans are associated with mutations in the BarA/SirA regulatory pathway.Cell host & microbe · 2024Article
- From intestine to beyond:Open biology · 2024Article
- Major primary bile salts repressFrontiers in microbiology · 2024Article
- Antibacterial and Antivirulence Activities of Acetate, Zinc Oxide Nanoparticles, and Vitamin C Against E. coli O157:H7 and P. aeruginosa.Current microbiology · 2023Article
- Mitigating the attachment of Salmonella Infantis on isolated poultry skin with cetylpyridinium chloride.PloS one · 2023Article
- The Assembly of Flagella in EnteropathogenicInternational journal of molecular sciences · 2022Article
- Potential of gut-derived short-chain fatty acids to control enteric pathogens.Frontiers in microbiology · 2022Review
- HilE is required for synergistic activation of SPI-1 gene expression in Salmonella enterica serovar Typhimurium.BMC microbiology · 2021Article
- Single Cell Analysis of Bistable Expression of Pathogenicity Island 1 and the Flagellar Regulon inMicroorganisms · 2021Article
- Regulatory protein HilD stimulates Salmonella Typhimurium invasiveness by promoting smooth swimming via the methyl-accepting chemotaxis protein McpC.Nature communications · 2021Article
- Two Tandem Mechanisms Control Bimodal Expression of the Flagellar Genes in Salmonella enterica.Journal of bacteriology · 2020Article
- Transcriptomic Analysis, Motility and Biofilm Formation Characteristics ofInternational journal of molecular sciences · 2020Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSalmonella enterica serovar Typhimurium is a common food-borne pathogen. S. enterica uses a type III secretion system encoded within Salmonella pathogenicity island 1 (SPI-1) to invade intestinal epithelial cells. A complex network of interacting transcription factors regulates SPI-1 gene expression. In addition, SPI-1 gene expression is coupled to flagellar gene expression. Both SPI-1 and flagellar gene expression are bistable, with co-existing populations of cells expressing and not expressing these genes. Previous work demonstrated that nutrients could be used to tune the fraction of cells expressing the flagellar genes. In the present study, we tested whether nutrients could also tune the fraction of cells expressing the SPI-1 genes through transcriptional crosstalk with the flagellar genes.
resultsNutrients alone were not found to induce SPI-1 gene expression. However, when the cells were also grown in the presence of acetate, the concentration of nutrients in the growth medium was able to tune the fraction of cells expressing the SPI-1 genes. During growth in nutrient-poor medium, acetate alone was unable to induce SPI-1 gene expression. These results demonstrate that acetate and nutrients synergistically activate SPI-1 gene expression. The response to acetate was governed by the BarA/SirA two-component system and the response to nutrients was governed by transcriptional crosstalk with the flagella system, specifically through the action of the flagellar regulator FliZ.
conclusionsAcetate and nutrients are capable of synergistically activating SPI-1 gene expression. In addition, these signals were found to tune the fraction of cells expressing the SPI-1 genes. The governing mechanism involves transcriptional crosstalk with the flagellar gene network. Collectively, these results further our understanding of SPI-1 gene regulation and provide the basis for future studies investigating this complex regulatory mechanism.
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