ArticleMikrochimica acta2019
Fluorometric determination for ofloxacin by using an aptamer and SYBR Green I.
Article in Mikrochimica acta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 57 citations in OpenAlex.
- Aptamer Engineering: Strategies for Discovering Functional Nucleic Acids for Next-Generation Diagnostics and Biosensing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Recent Advances of Fluorescent Aptasensors for the Detection of Antibiotics in Food.Biosensors · 2025Review
- Aptamer-Based Sensor for Rapid and Sensitive Detection of Ofloxacin in Meat Products.Sensors (Basel, Switzerland) · 2024Article
- Review
- Engineering constructed of high selectivity dexamethasone aptamer based on truncation and mutation technology.Frontiers in bioengineering and biotechnology · 2022Article
- A nanosol SERS/RRS aptamer assay of trace cobalt(ii) by covalent organic framework BtPD-loaded nanogold catalytic amplification.Nanoscale advances · 2021Article
- Detection of Single Nucleotide Polymorphisms by Fluorescence Embedded Dye SYBR Green I Based on Graphene Oxide.Frontiers in chemistry · 2021Article
- Investigation of a Truncated Aptamer for Ofloxacin Detection Using a Rapid FRET-Based Apta-Assay.Antibiotics (Basel, Switzerland) · 2020Article
- Ratiometric fluorescent nanoprobe based on CdTe/SiOMikrochimica acta · 2020Article
- An aptasensor strip-based colorimetric determination method for kanamycin using cellulose acetate nanofibers decorated DNA-gold nanoparticle bioconjugates.Mikrochimica acta · 2020Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
A fluorometric method is described for the determination of ofloxacin (OFL). It is based on the use of the fluorescent intercalator SYBR Green I (SG-I). The OFL-aptamer has G-quadruplex structures and can be recognized by SG-I. It results in strong fluorescence of SG-I. If OFL is present, OFL will bind to its aptamer to form stable complexes. This induces the despiralization of partial dsDNA regions, leads to changes in the structure of the aptamer. Thus, SG-I is released from the OFL-aptamer into solution. Hence, the fluorescence of SG-I drops. Fluorescence decreases linearly in the 1.1 to 200 nM OFL concentration range, and the limit of detection is 0.34 nM. The method shows good selectivity to much interference including analogues, hormones, pesticides. It is also effortless and fast with the times of measurement of <40 min. In addition, good recoveries of 91.3-119.0% were found for tap water, river water and artificial urine spiked with OFL with relative standard deviation (RSD) of ≤11.6%. Graphical abstract A sensitive fluorometric method is developed for ofloxacin (OFL) detection in aqueous samples based on the fluorescence intensity change of SYBR Green I (SG-I) with or without OFL.
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