Evidence map›Paper›PMID 31485767›Full record

ArticleJournal of cancer research and clinical oncology2019

Stress-triggered YAP1/SOX2 activation transcriptionally reprograms head and neck squamous cell carcinoma for the acquisition of stemness.

Hirofumi Omori, Kuniaki Sato, Takafumi Nakano, Takahiro Wakasaki, Satoshi Toh, Kenichi Taguchi, Takashi Nakagawa, Muneyuki Masuda

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cancer research and clinical oncology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.0field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Molecular function of Krüppel-like factor 7 in biology.Acta biochimica et biophysica Sinica · 2023
    Review
  3. Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. LINC01207 Predicts Poor Prognosis and Suppresses Cell Growth and Metastasis via Regulating GSK-3β/β-Catenin Signaling Pathway in Malignant Glioma.Medical science monitor : international medical journal of experimental and clinical research · 2020
    Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Hirofumi OmoriDepartment of Head and Neck Surgery, National Hospital Organization Kyushu Cancer Center, 3-1-1 Notame, Minamiku, Fukuoka, Fukuoka, 811-1395, Japan.
Kuniaki SatoDepartment of Head and Neck Surgery, National Hospital Organization Kyushu Cancer Center, 3-1-1 Notame, Minamiku, Fukuoka, Fukuoka, 811-1395, Japan.
Takafumi NakanoDepartment of Head and Neck Surgery, National Hospital Organization Kyushu Cancer Center, 3-1-1 Notame, Minamiku, Fukuoka, Fukuoka, 811-1395, Japan.
Takahiro WakasakiDepartment of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashiku, Fukuoka, Fukuoka, 812-8582, Japan.
Satoshi TohDepartment of Head and Neck Surgery, National Hospital Organization Kyushu Cancer Center, 3-1-1 Notame, Minamiku, Fukuoka, Fukuoka, 811-1395, Japan.
Kenichi TaguchiDepartment of Pathology, National Kyushu Cancer Center, 3-1-1 Notame, Minamiku, Fukuoka, Fukuoka, 811-1395, Japan.
Takashi NakagawaDepartment of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashiku, Fukuoka, Fukuoka, 812-8582, Japan.
Muneyuki MasudaDepartment of Head and Neck Surgery, National Hospital Organization Kyushu Cancer Center, 3-1-1 Notame, Minamiku, Fukuoka, Fukuoka, 811-1395, Japan. mmuneyuki@icloud.com.ORCID http://orcid.org/0000-0002-7479-8356
National Hospital Organization Kyushu Cancer Center · JPKyushu University · JP

Funding

MEXT/JSPS KAKENHI JP16K11255MEXT/JSPS KAKENHI JP16K11256
6 · The paper itself

Abstract

purposeThe clinical importance of cancer stem cells (CSCs) in head and neck squamous cell carcinoma (HNSCC) is well recognized. However, a reliable method for the detection of functioning CSC has not yet been established. We hypothesized that YAP1, a transcriptional coactivator, and SOX2, a master transcription factor of SCC, may cooperatively induce stemness through transcriptional reprogramming.

methodsWe immunohistochemically examined the expression of SOX2 and YAP1 in the CD44 variant 9 (CD44v9)-positive invasion front. A CSC-inducible module was identified through a combination of siRNAs and sphere formation assays. YAP1 and SOX2 interactions were analyzed in vitro.

resultsThe triple overexpression of SOX2, YAP1, and CD44v9 was significantly associated with poor prognosis. TCGA data revealed that the CSC-inducible module, which was related to EMT and angiogenesis, was significantly correlated with poor prognosis. The KLF7 expression, representatively chosen from the module, also correlated with poor prognosis and was essential for sphere formation and CSC propagation. Sphere stress-activated YAP1 enhanced SOX2 activity.

conclusionsThe stress-triggered activation of YAP1/SOX2 transcriptionally reprograms HNSCC for the acquisition of stemness. Triple SOX2, YAP1, and CD44v9 immunostaining assays may be useful for the selection of high-risk patients with functioning CSCs, and YAP1 targeting may lead to the development of a CSC-targeting therapy.

Indexed as

Stress, PhysiologicalTranscriptional ActivationAdaptor Proteins, Signal TransducingBiomarkersCell Line, TumorGene Expression ProfilingHumansImmunohistochemistryNeoplastic Stem CellsPhosphoproteinsRNA, Small InterferingSOXB1 Transcription FactorsSquamous Cell Carcinoma of Head and NeckTranscription FactorsYAP-Signaling ProteinsAdaptor Proteins, Signal TransducingBiomarkersPhosphoproteinsRNA, Small InterferingSOX2 protein, humanSOXB1 Transcription FactorsTranscription FactorsYAP1 protein, humanYAP-Signaling ProteinsCancer stem cellHead and neck squamous cell carcinomaKLF7SOX2YAP1

Identifiers

PMID31485767
PMCPMC11810275
OpenAlexW2971780389

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.