Evidence map›Paper›PMID 31485604›Full record

ArticleInternational journal of molecular medicine2019

Overexpression of miR‑200a‑3p promoted inflammation in sepsis‑induced brain injury through ROS‑induced NLRP3.

Jianhua Yu, Jinlong Chen, Hualing Yang, Sifang Chen, Zhanxiang Wang

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PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed, 1 pooled it
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 1 synthesis or guideline pooled it, 25 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Jianhua YuDepartment of ICU, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361000, P.R. China.
Jinlong ChenDepartment of ICU, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361000, P.R. China.
Hualing YangDepartment of Neurosurgery, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361000, P.R. China.
Sifang ChenDepartment of Neurosurgery, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361000, P.R. China.
Zhanxiang WangDepartment of Neurosurgery, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361000, P.R. China.
First Affiliated Hospital of Xiamen University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Sepsis, a systemic inflammatory response syndrome induced by infection, is a common complication of trauma, burns, postoperative infection and critical disease, and is characterized by an acute onset and high fatality rate. The aim of the present study was to explore the possible molecular mechanisms of microRNA‑200a‑3p (miRNA‑200a‑3p) on inflammation during sepsis. Reverse transcription‑quantitative PCR and gene microarray were used to measure the expression of miRNA‑200a‑3p. Tumor necrosis factor‑α, interleukin (IL)‑1β, IL‑6 and IL‑18 were searched by ELISA. The related proteins expression was measured using western blotting. The expression of miRNA‑200a‑3p was markedly higher in the sepsis model when compared with the normal control group. In addition, the expression of miRNA‑200a‑3p was upregulated by the miRNA‑200a‑3p plasmid in human brain microvascular endothelial cells treated with lipopolysaccharide, which further induced inflammation via the induction of NLR family pyrin domain containing 3 (NLRP3) and suppression of Kelch like ECH associated protein (Keap)‑1/nuclear factor erythroid 2 like 2 (Nrf2)/heme oxygenase (HO)‑1. The inhibition of Keap1/Nrf2/HO‑1 attenuated the effects of anti‑miRNA‑200a‑3p on inflammation. However, the inhibition of NLRP3 attenuated the effects of miRNA‑200a‑3p on inflammation. In conclusion, to the best of our knowledge, the results of the present study demonstrated for the first time that overexpression of miRNA‑200a‑3p promoted inflammation in sepsis‑induced brain injury through reactive oxygen species‑induced NLRP3.

Indexed as

AnimalsBrainBrain InjuriesCells, CulturedEndothelial CellsHumansInflammationKelch-Like ECH-Associated Protein 1MaleMiceMice, Inbred C57BLMicroRNAsNLR Family, Pyrin Domain-Containing 3 ProteinReactive Oxygen SpeciesSepsisSignal TransductionKelch-Like ECH-Associated Protein 1MicroRNAsMIRN200 microRNA, humanNLR Family, Pyrin Domain-Containing 3 ProteinNLRP3 protein, humanNlrp3 protein, mouseReactive Oxygen Species

Identifiers

PMID31485604
PMCPMC6777670
OpenAlexW2970251704

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.