ArticleScientific reports2019
A novel technique to prepare a single cell suspension of isolated quiescent human hepatic stellate cells.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- Protocol to maximize single-nuclei multiome library complexity for limited primary tissues via optimized sample preparation and library synthesis.STAR protocols · 2026Article
- Semi-automated isolation of parenchymal and non-parenchymal liver cells from mice and humans with enhanced stellate cell fraction.Cell & bioscience · 2026Article
- Challenges ofFrontiers in cell and developmental biology · 2025Review
- Oxytocin alleviates liver fibrosis via hepatic macrophages.JHEP reports : innovation in hepatology · 2024Article
- KIF18A inactivates hepatic stellate cells and alleviates liver fibrosis through the TTC3/Akt/mTOR pathway.Cellular and molecular life sciences : CMLS · 2024Article
- Developmental Changes of Duckling Liver and Isolation of Primary Hepatocytes.Animals : an open access journal from MDPI · 2023Article
- Application of Single-Cell RNA Sequencing in Ovarian Development.Biomolecules · 2022Review
- Using mass cytometry for the analysis of samples of the human airways.Frontiers in immunology · 2022Article
- Hepatic Stellate Cell: A Double-Edged Sword in the Liver.Physiological research · 2021Review
- Therapeutic targeting of STAT3 with small interference RNAs and antisense oligonucleotides embedded exosomes in liver fibrosis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Mitochondria-specific delivery system for targeted regulation of mitochondrial gene expression.STAR protocols · 2021Article
- Optimization of the isolation procedure and culturing conditions for hepatic stellate cells obtained from mouse.Bioscience reports · 2021Article
- Characterization of Immune Cells and Proinflammatory Mediators in the Pulmonary Environment.Journal of visualized experiments : JoVE · 2020Article
- Hepatic stellate cells - from past till present: morphology, human markers, human cell lines, behavior in normal and liver pathology.Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologieReview
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To explore a simple and easy-to-learn procedure for the isolation of human quiescent hepatic stellate cells (HSCs) that requires no advanced training. Thus reducing costs and increasing efficiency. This protocol will provide sufficient primary cells with minimal contaminants for future basic research on diseases associated with human HSCs. Normal liver tissues were isolated from patients undergoing hepatic hemangioma resection, and a single cell suspension of these tissues was prepared using the Gentle MACS tissue processor. By using this method, the difficulty of the procedure was reduced, fewer cells were lost during the preparation treatments, and the maximal activity of single cells was maintained. Following preparation of the cell suspension, the HSCs were further isolated using a Nycodenz density gradient. Cell viability was examined by trypan blue staining, and the purity of the quiescent human HSCs was determined by autofluorescence and oil red O staining. Activated and quiescent human HSCs were identified using immunofluorescence and Western blotting. The cell cycle distribution in activated and quiescent human HSCs was analyzed by flow cytometry.The recovery rate of the HSCs was approximately (2.1 ± 0.23) × 10
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