ArticleJournal of clinical microbiology2019
Evaluation of a Novel Multiplex High-Definition PCR Assay for Detection of Tick-Borne Pathogens in Whole-Blood Specimens.
Article in Journal of clinical microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- Improving Molecular Detection of Tick-Borne Pathogens in Citizen-Collected Ticks.Pathogens (Basel, Switzerland) · 2026Article
- Multiplexed, universal probe-based rare variant detection with USE-PCR.Scientific reports · 2025Article
- Evidence for the long-distance transport of ticks and tick-borne pathogens by human travellers to Texas, USA.Journal of travel medicine · 2025Article
- Antibodies to Anaplasma phagocytophilum in Patients with Human Granulocytic Anaplasmosis Confirmed by Both Polymerase Chain Reaction and Culture.The American journal of medicine · 2025Article
- Culture and other direct detection methods to diagnose human granulocytic anaplasmosis.American journal of clinical pathology · 2025Article
- Article
- Spotted fever diagnosis using molecular methods.Revista da Sociedade Brasileira de Medicina Tropical · 2024Review
- Developing a Prospective Gestational Lyme Disease Study.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Tick-Borne Co-Infections: Challenges in Molecular and Serologic Diagnoses.Pathogens (Basel, Switzerland) · 2023Review
- Multiplex High-Definition Polymerase Chain Reaction Assay for the Diagnosis of Tick-borne Infections in Children.Open forum infectious diseases · 2023Article
- A scoping review of zoonotic parasites and pathogens associated with abattoirs in Eastern Africa and recommendations for abattoirs as disease surveillance sites.Frontiers in public health · 2023Article
- Technical Evaluation of qPCR Multiplex Assays for the Detection ofMicroorganisms · 2022Article
- Current and Future Molecular Diagnostics of Tick-Borne Diseases in Cattle.Veterinary sciences · 2022Review
- Capture Sequencing Enables Sensitive Detection of Tick-Borne Agents in Human Blood.Frontiers in microbiology · 2022Article
- Tick-borne zoonoses and commonly used diagnostic methods in human and veterinary medicine.Parasitology research · 2021Review
- Technologies for Detection ofPathogens (Basel, Switzerland) · 2021Review
- Gap Analysis of the Habitat Interface of Ticks and Wildlife in Mexico.Pathogens (Basel, Switzerland) · 2021Review
- Seasonality of Acute Lyme Disease in Children.Tropical medicine and infectious disease · 2021Article
- Review
- Discovery and Surveillance of Tick-Borne Pathogens.Journal of medical entomology · 2021Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The prevalence of tick-borne infections has been steadily increasing in both number and geographic distribution in the United States and abroad. This increase, in conjunction with the continued recognition of novel pathogens transmitted by ticks, has made accurate diagnosis of these infections challenging. Mainstay serologic tests are insensitive during the acute phase of infection and are often cross-reactive with similar pathogenic and nonpathogenic organisms. Further, they are unable to reliably differentiate active versus past infection which can lead to misdiagnosis and incorrect understanding of the epidemiology and incidence of specific tick-borne pathogens. We evaluated a novel multiplexed high-definition PCR (HDPCR) Tickborne Panel (TBP) assay (ChromaCode, Carlsbad, CA) for the detection of nine tick-borne pathogens or groups associated with human illness. The HDPCR technology enables multiplex identification of multiple targets in a single fluorometric channel based on fluorescent signal modulation using a limiting probe design. A collection of 530 whole-blood specimens collected from patients being evaluated for tick-borne infections, in addition to a panel of 93 simulated specimens, were used to challenge the HDPCR TBP. The results were compared to a clinically validated traditional multiplexed PCR test with additional sequence analysis and clinical history collected to aid in resolving discrepancies. Among clinical specimens the TBP demonstrated 100% sensitivity for the identification of
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.