ArticleBMC veterinary research2019
Tropism of Newcastle disease virus strains for chicken neurons, astrocytes, oligodendrocytes, and microglia.
Article in BMC veterinary research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 46 citations in OpenAlex.
- Comparative evaluation of oncolytic viruses reveals opposing preferences for glioblastoma subtypes.Molecular therapy. Oncology · 2026Article
- Chicken microRNA 26a-5p regulates replication of Newcastle disease virus by direct targeting of the viral polymerase.Virology journal · 2026Article
- Characterizing Host microRNA: Virus Interactions ofViruses · 2024Article
- Detection and differentiation of low virulence and virulent Orthoavulavirus javaense using a molecular beacon with RT-LAMP.Scientific reports · 2024Article
- Article
- Protective effectiveness of two vaccination schemes against the prevalent Egyptian strain of Newcastle disease virus genotype VII.Open veterinary journal · 2024Article
- Role of Macrophages in the Pathogenesis of Genotype VII Newcastle Disease Virus in Chickens.Animals : an open access journal from MDPI · 2023Article
- Immune Functions of Astrocytes in Viral Neuroinfections.International journal of molecular sciences · 2023Review
- Rescue of an enterotropic Newcastle disease virus strain ZM10 from cloned cDNA and stable expressing an inserted foreign gene.BMC biotechnology · 2022Article
- Review detection of Newcastle disease virus.Frontiers in veterinary science · 2022Review
- Pathogenesis of Velogenic Genotype VII.1.1 Newcastle Disease Virus Isolated from Chicken in Egypt via Different Inoculation Routes: Molecular, Histopathological, and Immunohistochemical Study.Animals : an open access journal from MDPI · 2021Article
- Article
- Sequential Pathology of a Genotype XIII Newcastle Disease Virus from Bangladesh in Chickens on Experimental Infection.Pathogens (Basel, Switzerland) · 2020Article
- Newcastle disease virus induces testicular damage and disrupts steroidogenesis in specific pathogen free roosters.Veterinary research · 2020Article
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Authors and funding
10 authors at 2 institutions in 3 countries.
Funding
Abstract
backgroundNewcastle disease (ND), which is caused by infections of poultry species with virulent strains of Avian orthoavulavirus-1, also known as avian paramyxovirus 1 (APMV-1), and formerly known as Newcastle disease virus (NDV), may cause neurological signs and encephalitis. Neurological signs are often the only clinical signs observed in birds infected with neurotropic strains of NDV. Experimental infections have shown that the replication of virulent NDV (vNDV) strains is in the brain parenchyma and is possibly confined to neurons and ependymal cells. However, little information is available on the ability of vNDV strains to infect subset of glial cells (astrocytes, oligodendrocytes, and microglia). The objective of this study was to evaluate the ability of NDV strains of different levels of virulence to infect a subset of glial cells both in vitro and in vivo. Thus, neurons, astrocytes and oligodendrocytes from the brains of day-old White Leghorn chickens were harvested, cultured, and infected with both non-virulent (LaSota) and virulent, neurotropic (TxGB) NDV strains. To confirm these findings in vivo, the tropism of three vNDV strains with varying pathotypes (SA60 [viscerotropic], TxGB [neurotropic], and Tx450 [mesogenic]) was assessed in archived formalin-fixed material from day-old chicks inoculated intracerebrally.
resultsDouble immunofluorescence for NDV nucleoprotein and cellular markers showed that both strains infected at least 20% of each of the cell types (neurons, astrocytes, and oligodendrocytes). At 24 h post-inoculation, TxGB replicated significantly more than LaSota. Double immunofluorescence (DIFA) with markers for neurons, astrocytes, microglia, and NDV nucleoprotein detected the three strains in all three cell types at similar levels.
conclusionThese data indicate that similar to other paramyxoviruses, neurons and glial cells (astrocytes, oligodendrocytes, and microglia) are susceptible to vNDV infection, and suggest that factors other than cellular tropism are likely the major determinant of the neurotropic phenotype.
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