Evidence map›Paper›PMID 31482666›Full record

ArticleChembiochem : a European journal of chemical biology2020

Engineering of a Red Fluorogenic Protein/Merocyanine Complex for Live-Cell Imaging.

Elizabeth M Santos, Tetyana Berbasova, Wenjing Wang, Rahele Esmatpour Salmani, Wei Sheng, Chrysoula Vasileiou, James H Geiger, Babak Borhan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Photoisomerization detected in a fully wavelength-tunable rhodopsin mimic system.Acta crystallographica. Section D, Structural biology · 2026
    Article
  2. Article
  3. Article
  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Elizabeth M SantosDepartment of Chemistry, Michigan State University, 578 S. Shaw Lane, East Lansing, MI, 48824, USA.
Tetyana BerbasovaDepartment of Chemistry, Michigan State University, 578 S. Shaw Lane, East Lansing, MI, 48824, USA.
Wenjing WangDepartment of Chemistry, Michigan State University, 578 S. Shaw Lane, East Lansing, MI, 48824, USA.
Rahele Esmatpour SalmaniDepartment of Chemistry, Michigan State University, 578 S. Shaw Lane, East Lansing, MI, 48824, USA.
Wei ShengDepartment of Chemistry, Michigan State University, 578 S. Shaw Lane, East Lansing, MI, 48824, USA.
Chrysoula VasileiouDepartment of Chemistry, Michigan State University, 578 S. Shaw Lane, East Lansing, MI, 48824, USA.
James H GeigerDepartment of Chemistry, Michigan State University, 578 S. Shaw Lane, East Lansing, MI, 48824, USA.
Babak BorhanDepartment of Chemistry, Michigan State University, 578 S. Shaw Lane, East Lansing, MI, 48824, USA.ORCID 0000-0002-3193-0732

Funding

Protein/Chromphore Interactions via Protein Design: Interrogation and ApplicationR01GM101353 · NIGMS · MICHIGAN STATE UNIVERSITY · PI BORHAN, BABAK, GEIGER, JAMES H · 2013 to 2022
$2.6M
NIGMS NIH HHS R01 GM101353
6 · The paper itself

Abstract

A reengineered human cellular retinol binding protein II (hCRBPII), a 15-kDa protein belonging to the intracellular lipid binding protein (iLBP) family, generates a highly fluorescent red pigment through the covalent linkage of a merocyanine aldehyde to an active site lysine residue. The complex exhibits "turn-on" fluorescence, due to a weakly fluorescent aldehyde that "lights up" with subsequent formation of a strongly fluorescent merocyanine dye within the binding pocket of the protein. Cellular penetration of merocyanine is rapid, and fluorophore maturation is nearly instantaneous. The hCRBPII/merocyanine complex displays high quantum yield, low cytotoxicity, specificity in labeling organelles, and compatibility in both cancer cell lines and yeast cells. The hCRBPII/merocyanine tag is brighter than most common red fluorescent proteins.

Indexed as

AnimalsBenzopyransChlorocebus aethiopsCOS CellsFluorescent DyesHeLa CellsHumansIndolesRetinol-Binding Proteins, CellularSaccharomyces cerevisiaeBenzopyransFluorescent DyesIndolesmerocyanineRBP2 protein, humanRetinol-Binding Proteins, CellularhCRBPIIimagingmerocyanineprotein engineeringred fluorescence

Identifiers

PMID31482666
PMCPMC7379159

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.