ArticleNeurotoxicity research2020
Neurotoxic Effect of Fipronil in Neuroblastoma SH-SY5Y Cell Line.
Article in Neurotoxicity research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed, 18 citations in OpenAlex.
- Neuroprotective efficacy of hentriacontane against rotenone-induced apoptosis in SH-SY5Y cells: In silico and in vitro evidence of GSK3β association.Naunyn-Schmiedeberg's archives of pharmacology · 2026Article
- VDAC1 Protein Regulation of Oxidative Damage and Mitochondrial Dysfunction-Mediated Cytotoxicity by Silica Nanoparticles in SH-SY5Y Cells.Molecular neurobiology · 2023Article
- New Insights into the Effect of Fipronil on the Soil Bacterial Community.Microorganisms · 2022Article
- Flusilazole Induced Cytotoxicity and Inhibition of Neuronal Growth in Differentiated SH-SY5Y Neuroblastoma Cells by All-Trans-Retinoic Acid (Atra)Turkish journal of pharmaceutical sciences · 2021Article
- Ameliorative Role of Cerium Oxide Nanoparticles Against Fipronil Impact on Brain Function, Oxidative Stress, and Apoptotic Cascades in Albino Rats.Frontiers in neuroscience · 2021Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Fipronil is a broad-spectrum insecticide belonging to the phenyl pyrazole chemical family. Fipronil disrupts gamma-aminobutyric acid (GABA) receptors in the central nervous system, thereby blocking GABA-gated chloride channels. Neurotoxic symptoms of fipronil poisoning in humans are typically associated with the antagonism of central GABA receptors. In this study, the cytotoxic effects of fipronil in SH-SY5Y neuroblastoma cell line, the human neural cell line, were investigated. SH-SY5Y cells were exposed to 125, 250, and 500 μM fipronil doses and incubated for 24 and 48 h, and its neurotoxic effect was examined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide) test, lactate dehydrogenase (LDH) test, trypan blue dye exclusion test, and neutral red uptake assay. Acridine orange-propidium iodide and DAPI (4',6-diamidino-2-phenylindole dihydrochloride) staining methods were also used to determine the type of cell death. In addition, neurodegenerative changes were also examined by immunofluorescence staining of the cytoskeleton. The amount of neurofilament heavy chain and the degree of phosphorylation of neurofilament subunits were investigated to determine the effect of fipronil on the axonal viability, and we observed a reduction in the amount of neurofilament heavy chain, speculating disruption of the axonal transport leading to cell death. According to our results, we may suggest that fipronil caused cytotoxicity in SH-SY5Y cells.
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Registered trials
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