ArticleFrontiers in behavioral neuroscience2019
An Automated Assay System to Study Novel Tank Induced Anxiety.
Article in Frontiers in behavioral neuroscience, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Modulation of Caffeine-Induced Behavioral Alterations by N-Acetylcysteine in a Pharmacological Challenge Model.Pharmaceuticals (Basel, Switzerland) · 2026Article
- Zebrafish frk Gene Knockout Shows Social Impairments Relevant to Autism and Delays Brain Development by Increasing Cell Proliferation.Autism research : official journal of the International Society for Autism Research · 2026Article
- Sex-specific neurological dysregulation may underpin distinctly different male and female behavioural phenotypes in a zebrafish model of autism spectrum disorder.Behavioral and brain functions : BBF · 2026Article
- HealthAge: evaluation of intrinsic capacity changes in humans, mice, and killifish to explore the biology of aging.GeroScience · 2026Review
- Stress Coping Style Alters Functional Brain Network Activity to Acute Stressor.bioRxiv : the preprint server for biology · 2025Article
- Complex Sound Discrimination in Zebrafish: Auditory Learning Within a Novel "Go/Go" Decision-Making Paradigm.Animals : an open access journal from MDPI · 2025Article
- Distinct Swimming Behavioral Phenotypes Following Serotonin and Dopamine Transporter Modulation in the Adult Zebrafish Novel Tank Diving Test (NTT).Pharmaceuticals (Basel, Switzerland) · 2025Article
- Inhibition mediated by group III metabotropic glutamate receptors regulates habenula activity and defensive behaviors.Nature communications · 2025Article
- From computational screening to zebrafish testing: repurposing of doxazosin, donepezil, and dolutegravir for neuroprotective potential in Alzheimer's disease.Naunyn-Schmiedeberg's archives of pharmacology · 2025Article
- Housing and husbandry factors affecting zebrafish novel tank test responses: a global multi-laboratory study.Lab animal · 2025Article
- The Impact of Oxytocin on Stimulus Discrimination of Zebrafish Albino and Non-Albino Models.International journal of molecular sciences · 2025Article
- Neuroprotective Potential ofBiomolecules · 2025Article
- Refinement of the novel tank diving test: toward standardized and robust analysis of anxiety-like behavior in zebrafish.Frontiers in behavioral neuroscience · 2025Article
- The Roles of Histamine Receptor 1 (hrh1) in Neurotransmitter System Regulation, Behavior, and Neurogenesis in Zebrafish.Molecular neurobiology · 2023Article
- Zebrafish Models of Rare Neurological Diseases like Spinocerebellar Ataxias (SCAs): Advantages and Limitations.Biology · 2023Review
- Assessing Drug Administration Techniques in Zebrafish Models of Neurological Disease.International journal of molecular sciences · 2023Review
- Article
- Assessing Chronodisruption Distress in Goldfish: The Importance of Multimodal Approaches.Animals : an open access journal from MDPI · 2023Article
- Behavioral Study of 3- and 5-Halocytisine Derivatives in Zebrafish Using the Novel Tank Diving Test (NTT).International journal of molecular sciences · 2023Article
- Housing Conditions Affect Adult Zebrafish (Animals : an open access journal from MDPI · 2023Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
New environments are known to be anxiogenic initially for many animals including the zebrafish. In the zebrafish, a novel tank diving (NTD) assay for solitary fish has been used extensively to model anxiety and the effect of anxiolytics. However, studies can differ in the conditions used to perform this assay. Here, we report the development of an efficient, automated toolset and optimal conditions for effective use of this assay. Applying these tools, we found that two important variables in previous studies, the direction of illumination of the novel tank and the age of the subject fish, both influence endpoints commonly measured to assess anxiety. When tanks are illuminated from underneath, several parameters such as the time spent at the bottom of the tank, or the transitions to the top half of the tank become poor measures of acclimation to the novel environment. Older fish acclimate faster to the same settings. The size of the novel tank and the intensity of the illuminating light can also influence acclimation. Among the parameters measured, reduction in the frequency of erratic swimming (darting) is the most reliable indicator of anxiolysis. Open source pipeline for automated data acquisition and systematic analysis generated here and available to other researchers will improve accessibility and uniformity in measurements. They can also be directly applied to study other fish. As this assay is commonly used to model anxiety phenotype of neuropsychiatric ailments in zebrafish, we expect our tools will further aid comparative and meta-analyses.
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