ArticleScientific reports2019
Efficient generation of Knock-in/Knock-out marmoset embryo via CRISPR/Cas9 gene editing.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 64 citations in OpenAlex.
- Generation of marmoset monkeys with a non-mosaic disruption of the OTOF gene as a model of human deafness.Nature communications · 2026Article
- Article
- Higher dopamine D1 receptor expression in prefrontal parvalbumin neurons underlies higher distractibility in marmosets versus macaques.Communications biology · 2025Article
- Non-human primate: the new frontier model of female reproductive engineering.Frontiers in bioengineering and biotechnology · 2025Review
- Development of a Marmoset Apparatus for Automated Pulling to study cooperative behaviors.eLife · 2024Article
- Development of a Marmoset Apparatus for Automated Pulling (MarmoAAP) to Study Cooperative Behaviors.bioRxiv : the preprint server for biology · 2024Article
- Production of a heterozygous exon skipping model of common marmosets using gene-editing technology.Lab animal · 2024Article
- Marmosets as models of infectious diseases.Frontiers in cellular and infection microbiology · 2024Review
- An expedition in the jungle of pluripotent stem cells of non-human primates.Stem cell reports · 2023Review
- The neurobiology of vocal communication in marmosets.Annals of the New York Academy of Sciences · 2023Review
- Generation of a tyrosine hydroxylase-2A-Cre knockin non-human primate model by homology-directed-repair-biased CRISPR genome editing.Cell reports methods · 2023Article
- The history, use, and challenges of therapeutic somatic cell and germline gene editing.Fertility and sterility · 2023Review
- Evaluation and comparison of pharmacokinetic profiles and safety of two extended-release buprenorphine formulations in common marmosets (Callithrix jacchus).Scientific reports · 2023Article
- The common marmoset in biomedical research: experimental disease models and veterinary management.Experimental animals · 2023Review
- Research and Therapeutic Approaches in Stem Cell Genome Editing by CRISPR Toolkit.Molecules (Basel, Switzerland) · 2023Review
- KIF4 regulates neuronal morphology and seizure susceptibility via the PARP1 signaling pathway.The Journal of cell biology · 2023Article
- Genetically encoded fluorescent sensors for imaging neuronal dynamics in vivo.Journal of neurochemistry · 2023Review
- Corneal endothelial transplantation from bench to bedside: A review of animal models and their translational value for therapeutic development.Experimental eye research · 2022Review
- The versatile electric condition in mouse embryos for genome editing using a three-step square-wave pulse electroporator.Experimental animals · 2022Article
- Flexible auditory training, psychophysics, and enrichment of common marmosets with an automated, touchscreen-based system.Nature communications · 2022Article
Corrections and comments
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Authors and funding
13 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetically modified nonhuman primates (NHP) are useful models for biomedical research. Gene editing technologies have enabled production of target-gene knock-out (KO) NHP models. Target-gene-KO/knock-in (KI) efficiency of CRISPR/Cas9 has not been extensively investigated in marmosets. In this study, optimum conditions for target gene modification efficacies of CRISPR/mRNA and CRISPR/nuclease in marmoset embryos were examined. CRISPR/nuclease was more effective than CRISPR/mRNA in avoiding mosaic genetic alteration. Furthermore, optimal conditions to generate KI marmoset embryos were investigated using CRISPR/Cas9 and 2 different lengths (36 nt and 100 nt) each of a sense or anti-sense single-strand oligonucleotide (ssODN). KIs were observed when CRISPR/nuclease and 36 nt sense or anti-sense ssODNs were injected into embryos. All embryos exhibited mosaic mutations with KI and KO, or imprecise KI, of c-kit. Although further improvement of KI strategies is required, these results indicated that CRISPR/Cas9 may be utilized to produce KO/KI marmosets via gene editing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.