ArticleActa neuropathologica communications2019
Optimization of ultrastructural preservation of human brain for transmission electron microscopy after long post-mortem intervals.
Article in Acta neuropathologica communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Can MRI measure myelin? Systematic review, qualitative assessment, and meta-analysis of studies validating microstructural imaging with myelin histology.NeuroImage · 2021Pooled it
- Ultrastructural analysis of human uncinate fasciculus with coherent anti-Stokes Raman spectroscopy.Biophotonics discovery · 2026Article
- Comparison of Freeze-drying and Gradient Dehydration Treatment on X-ray Imaging for Three-dimensional Reconstruction of Early Human Embryonic Brain Samples.Neuroscience bulletin · 2026Article
- Digging Deep into Alzheimer Disease: How Electron Microscopy Helps Elucidating Its Pathogenesis.Cellular and molecular neurobiology · 2026Review
- Volume electron microscopy reveals 3D synaptic nanoarchitecture in postmortem human prefrontal cortex.iScience · 2025Article
- Harnessing brain-derived extracellular vesicles to support RDoC-based drug development.Neuroscience applied · 2025Review
- Preservation of cellular structure via immersion fixation in brain banking.Free neuropathology · 2025Article
- Evaluating ultrastructural preservation quality in banked brain tissue.Free neuropathology · 2025Article
- Morphological and Immunohistochemical Changes in Progressive Postmortem Autolysis of the Murine Brain.Animals : an open access journal from MDPI · 2024Article
- Ultrastructure of human brain tissue vitrified from autopsy revealed by cryo-ET with cryo-plasma FIB milling.Nature communications · 2024Article
- A Standardized Protocol for the Safe Retrieval of Infectious Postmortem Human Brain for Studying Whole-Brain Pathology.The American journal of forensic medicine and pathology · 2023Article
- Native ultrastructure of fresh human brain vitrified directly from autopsy revealed by cryo-electron tomography with cryo-plasma focused ion beam milling.bioRxiv : the preprint server for biology · 2023Article
- Autolysis Affects the Iron Cargo of Ferritins in Neurons and Glial Cells at Different Rates in the Human Brain.Cellular and molecular neurobiology · 2023Article
- Article
- Microglia in Human Postmortem Brain Samples: Quantitative Ultrastructural Analysis of Scanning Electron Microscopy Images.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Postmortem changes in brain cell structure: a review.Free neuropathology · 2023Article
- Digging Deeper: Advancements in Visualization of Inhibitory Synapses in Neurodegenerative Disorders.International journal of molecular sciences · 2021Review
- Simple method of thawing cryo-stored samples preserves ultrastructural features in electron microscopy.Histochemistry and cell biology · 2021Article
- Perisomatic innervation and neurochemical features of giant pyramidal neurons in both hemispheres of the human primary motor cortex.Brain structure & function · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors.
Funding
Abstract
Electron microscopy (EM) provides the necessary resolution to visualize the finer structures of nervous tissue morphology, which is important to understand healthy and pathological conditions in the brain. However, for the interpretation of the micrographs the tissue preservation is crucial. The quality of the tissue structure is mostly influenced by the post mortem interval (PMI), the time of death until the preservation of the tissue. Therefore, the aim of this study was to optimize the preparation-procedure for the human frontal lobe to preserve the ultrastructure as well as possible despite the long PMIs. Combining chemical pre- and post-fixation with cryo-fixation and cryo-substitution ("hybrid freezing"), it was possible to improve the preservation of the neuronal profiles of human brain samples compared to the "standard" epoxy resin embedding method. In conclusion short PMIs are generally desirable but up to a PMI of 16 h the ultrastructure can be preserved on an acceptable level with a high contrast using the "hybrid freezing" protocol described here.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.