Evidence map›Paper›PMID 31480202›Full record

ArticleAsian-Australasian journal of animal sciences2020

MiR-26a promotes apoptosis of porcine granulosa cells by targeting the 3β-hydroxysteroid-Δ24-reductase gene.

Xiaodong Zhang, Qiangqiang Tao, Jinnan Shang, Yiliang Xu, Liang Zhang, Yingchun Ma, Weihua Zhu, Min Yang, Yueyun Ding, Zongjun Yin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Asian-Australasian journal of animal sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.4field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Xiaodong ZhangCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
Qiangqiang TaoCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
Jinnan ShangCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
Yiliang XuCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
Liang ZhangCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
Yingchun MaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
Weihua ZhuCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
Min YangCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
Yueyun DingCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
Zongjun YinCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
Anhui Agricultural University · CN

Funding

Anhui Provincial Science and Technology Major Project 17030701061National Key R&D Program of China 2017YFD0600805National Natural Science Foundation of China 31972531,31402037Open Fund of Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding 2017003
6 · The paper itself

Abstract

objectiveApoptosis of ovarian granulosa cells (GCs) affects mammalian follicular development and fecundity. This study aimed to explore the regulatory relationship between microRNA-26a (miR-26a) and the 3β-hydroxysteroid-Δ24-reductase gene (DHCR24) gene in porcine follicular granular cells (pGCs), and to provide empirical data for the development of methods to improve the reproductive capacity of pigs.

methodsThe pGCs were transfected with miR-26a mimic, miR-26a inhibitor and DHCR24-siRNA in vitro. The cell apoptosis rate of pGCs was detected by the flow cytometry. The secretion levels of estradiol (E2) and progesterone (P) in pGCs were detected by enzymelinked immunosorbent assay. Double luciferase validation system was used to detect the binding sites between miR-26a and DHCR24 3'-UTR region. Qualitative real-time polymerase chain reaction and Western blotting were used to verify the DHCR24 mRNA and protein expression in pGCs, respectively, after transfecting with miR-26a mimic and miR-26a inhibitor.

resultsResults showed that enhancement of miR-26a promoted apoptosis, and inhibited E2 and P secretion in pGCs. Meanwhile, inhibition of DHCR24 also upregulated the Caspase-3 expression, reduced the BCL-2 expression, promoted pGCs apoptosis, and inhibited E2 and P secretion in pGCs. There were the binding sites of miR-26a located within DHCR24 3'-UTR. Up-regulation of miR-26a inhibited DHCR24 mRNA and protein expression in pGCs.

conclusionThis study demonstrates that miR-26a can promote cell apoptosis and inhibit E2 and P secretion by inhibiting the expression of DHCR24 in pGCs.

Indexed as

3β-hydroxysteroid-Δ24-reductase (DHCR24)ApoptosisGranulosa CellsmiR-26aPig

Identifiers

PMID31480202
PMCPMC7054607
OpenAlexW2953908751

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.